Back to Journals » Clinical Ophthalmology » Volume 4

Correlation between the matrix metalloproteinase-9 activity and chondroitin sulfate concentrations in tear fluid after laser in situ keratomileusis

Authors Muto T , Nishio M, Matsumoto Y, Arai K

Published 20 July 2010 Volume 2010:4 Pages 823—828

DOI https://doi.org/10.2147/OPTH.S11924

Review by Single anonymous peer review

Peer reviewer comments 3



Tetsuya Mutoh, Masaya Nishio, Yukihiro Matsumoto, Kiyomi Arai
Department of Ophthalmology, Dokkyo Medical University Koshigaya Hospital, Koshigaya, Saitama, Japan

Aims: The aim of the present study was to evaluate the correlation between matrix ­metalloproteinase-9 (MMP-9) and chondroitin sulfate (CS) concentrations in human tear fluid following laser in situ keratomileusis (LASIK).

Methods: Twelve eyes from six patients who had no ocular complaints except for ­refractive errors, and who had undergone LASIK, were enrolled in this study. We measured the ­concentrations of chondroitin 4 sulfate (C4S), chondroitin 6 sulfate (C6S), and MMP-9 activity with an enzyme-linked immunosorbent assay and an enzyme immunocapture activity assay preoperatively and postoperatively on days 1 and 4, week 1, and at 1 and 3 months.

Results: Although the preoperative MMP-9 activity and the C4S concentration were highly correlated (r = 0.900; P < 0.001), they were not postoperatively correlated at month 1. Although the preoperative MMP-9 activity and the C6S concentration were highly ­correlated (r = 0.885; P < 0.001), they were not postoperatively correlated at either week 1 or months 1 and 3.

Conclusions: The correlation appeared to be collapsed by LASIK, and it did not recover to the preoperative score at 3 months post-surgery. Our study indicates that the corneal wound healing was not terminated at 3 months following LASIK.

Keywords: preoperative correlation, postoperative correlation, wound healing, refractive errors, human cornea

Creative Commons License © 2010 The Author(s). This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.