Back to Journals » Infection and Drug Resistance » Volume 5

Conjunctival sac bacterial flora isolated prior to cataract surgery

Authors Suto C, Morinaga M, Yagi T, Tsuji C, Toshida H

Received 4 November 2011

Accepted for publication 19 December 2011

Published 24 January 2012 Volume 2012:5 Pages 37—41

DOI https://doi.org/10.2147/IDR.S27937

Review by Single-blind

Peer reviewer comments 3

Chikako Suto1,2, Masahiro Morinaga1,2, Tomoko Yagi1,2, Chieko Tsuji3, Hiroshi Toshida4
1Department of Ophthalmology, Saiseikai Kurihashi Hospital, Saitama; 2Department of Ophthalmology, Tokyo Women's Medical University, Tokyo; 3Department of Clinical Laboratory, Saiseikai Kurihashi Hospital, Saitama; 4Department of Ophthalmology, Juntendo University Shizuoka Hospital, Izunokuni, Shizuoka, Japan

Objective: To determine the trends of conjunctival sac bacterial flora isolated from patients prior to cataract surgery.
Subjects and methods: The study comprised 579 patients (579 eyes) who underwent cataract surgery. Specimens were collected by lightly rubbing the inferior palpebral conjunctival sac with a sterile cotton swab 2 weeks before surgery, and then cultured for isolation of bacteria and antimicrobial sensitivity testing. The bacterial isolates and percentage of drug-resistant isolates were compared among age groups and according to whether or not patients had diabetes mellitus, hyperlipidemia, dialysis therapy, oral steroid use, dry eye syndrome, or allergic conjunctivitis.
Results: The bacterial isolation rate was 39.2%. There were 191 strains of Gram-positive cocci, accounting for the majority of all isolates (67.0%), among which methicillin-sensitive coagulase-negative staphylococci was the most frequent (127 strains, 44.5%), followed by methicillin-resistant coagulase-negative staphylococci (37 strains, 12.7%). All 76 Gram-positive bacillary isolates (26.7%) were from the genus Corynebacterium. Among the 16 Gram-negative bacillary isolates (5.9%), the most frequent was Escherichia coli (1.0%). The bacterial isolation rate was higher in patients >60 years old, and was lower in patients with dry eye syndrome, patients under topical treatment for other ocular disorders, and patients with hyperlipidemia. There was no significant difference in bacterial isolation rate with respect to the presence/absence of diabetes mellitus, steroid therapy, dialysis, or a history of allergic conjunctivitis. Methicillin-resistant coagulase-negative staphylococci showed a significantly higher detection rate in diabetic patients than nondiabetic patients (20.3% versus 7.0%, P < 0.05). The percentage of all isolates resistant to levofloxacin, cefmenoxime, and tobramycin was 14.0%, 15.2%, and 17.9%, respectively, with no significant differences among these drugs.
Conclusion: The high bacterial isolation rate in patients >60 years old and the high methicillin-resistant coagulase-negative staphylococci isolation rate in patients with diabetes are important to consider for prevention of perioperative infections.

Keywords: endophthalmitis, cataract surgery, conjunctival sac, bacterial flora, diabetes mellitus

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]

 

Other articles by this author:

Efficacy and safety of retinol palmitate ophthalmic solution in the treatment of dry eye: a Japanese Phase II clinical trial

Toshida H, Funaki T, Ono K, Tabuchi N, Watanabe S, Seki T, Otake H, Kato T, Ebihara N, Murakami A

Drug Design, Development and Therapy 2017, 11:1871-1879

Published Date: 23 June 2017

Visual outcome after emergency surgery for open globe eye injury in Japan

Toride A, Toshida H, Matsui A, Matsuzaki Y, Honda R, Ohta T, Murakami A

Clinical Ophthalmology 2016, 10:1731-1736

Published Date: 8 September 2016

Persistent corneal epithelial defect responding to rebamipide ophthalmic solution in a patient with diabetes

Hayashi Y, Toshida H, Matsuzaki Y, Matsui A, Ohta T

International Medical Case Reports Journal 2016, 9:113-116

Published Date: 10 May 2016

Prevalence of drug resistance and culture-positive rate among microorganisms isolated from patients with ocular infections over a 4-year period

Shimizu Y, Toshida H, Honda R, Matsui A, Ohta T, Asada Y, Murakami A

Clinical Ophthalmology 2013, 7:695-702

Published Date: 9 April 2013

Efficacy of retinol palmitate eye drops for dry eye in rabbits with lacrimal gland resection

Odaka A, Toshida H, Ohta T, Tabuchi N, Koike D, Suto C, Murakami A

Clinical Ophthalmology 2012, 6:1585-1593

Published Date: 1 October 2012

Multipurpose soft contact lens care in Japan

Toshida H, Kadota Y, Suto C, Ohta T, Murakami A

Clinical Ophthalmology 2012, 6:139-144

Published Date: 18 January 2012

Effect of long-term treatment with eyedrops for glaucoma on conjunctival bacterial flora

Honda R, Toshida H, Suto C, Fujimaki T, Kimura T, Ohta T, Murakami A

Infection and Drug Resistance 2011, 4:191-196

Published Date: 20 October 2011

Bifocal contact lenses: History, types, characteristics, and actual state and problems

Hiroshi Toshida, Kozo Takahashi, Kazushige Sado, Atsushi Kanai, Akira Murakami

Clinical Ophthalmology 2008, 2:869-877

Published Date: 5 December 2008

Readers of this article also read:

Emerging and future therapies for hemophilia

Carr ME, Tortella BJ

Journal of Blood Medicine 2015, 6:245-255

Published Date: 3 September 2015

Methacrylic-based nanogels for the pH-sensitive delivery of 5-Fluorouracil in the colon

Ashwanikumar N, Kumar NA, Nair SA, Kumar GS

International Journal of Nanomedicine 2012, 7:5769-5779

Published Date: 15 November 2012

A novel preparation method for silicone oil nanoemulsions and its application for coating hair with silicone

Hu Z, Liao M, Chen Y, Cai Y, Meng L, Liu Y, Lv N, Liu Z, Yuan W

International Journal of Nanomedicine 2012, 7:5719-5724

Published Date: 12 November 2012

Cross-linked acrylic hydrogel for the controlled delivery of hydrophobic drugs in cancer therapy

Deepa G, Thulasidasan AK, Anto RJ, Pillai JJ, Kumar GS

International Journal of Nanomedicine 2012, 7:4077-4088

Published Date: 27 July 2012

Crystallization after intravitreal ganciclovir injection

Pitipol Choopong, Nattaporn Tesavibul, Nattawut Rodanant

Clinical Ophthalmology 2010, 4:709-711

Published Date: 14 July 2010