Concentration-dependent effects of fullerenol on cultured hippocampal neuron viability

Ying-ying Zha; Bo Yang; Ming-liang Tang; Qiu-chen Guo; Ju-tao Chen; Long-ping Wen; Ming Wang

doi:10.2147/IJN.S30934

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Original Research

Concentration-dependent effects of fullerenol on cultured hippocampal neuron viability

Authors Zha YY, Yang B, Tang ML, Guo QC, Chen JT, Wen LP, Wang M

Received 16 February 2012

Accepted for publication 17 April 2012

Published 29 June 2012 Volume 2012:7 Pages 3099—3109

DOI https://doi.org/10.2147/IJN.S30934

Review by Single-blind

Peer reviewer comments 2

Ying-ying Zha,¹ Bo Yang,¹ Ming-liang Tang,² Qiu-chen Guo,¹ Ju-tao Chen,¹ Long-ping Wen,³ Ming Wang¹
¹CAS Key Laboratory of Brain Function and Disease, School of Life Sciences, University of Science and Technology of China, Hefei, ²Suzhou Institute of NanoTech and NanoBionics, Chinese Academy of Sciences, Suzhou, ³Laboratory of Nano-biology, School of Life Sciences, University of Science and Technology of China, Hefei, People's Republic of China

Background: Recent studies have shown that the biological actions and toxicity of the water-soluble compound, polyhydroxyfullerene (fullerenol), are related to the concentrations present at a particular site of action. This study investigated the effects of different concentrations of fullerenol on cultured rat hippocampal neurons.
Methods and results: Fullerenol at low concentrations significantly enhanced hippocampal neuron viability as tested by MTT assay and Hoechst 33342/propidium iodide double stain detection. At high concentrations, fullerenol induced apoptosis confirmed by Comet assay and assessment of caspase proteins.
Conclusion: These findings suggest that fullerenol promotes cell death and protects against cell damage, depending on the concentration present. The concentration-dependent effects of fullerenol were mainly due to its influence on the reduction-oxidation pathway.

Keywords: fullerenol, nanomaterial, neurotoxicity, neuroprotection, hippocampal neuron

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