Back to Journals » International Journal of Nanomedicine » Volume 10 » Issue 1

Comparative assessment of the apoptotic potential of silver nanoparticles synthesized by Bacillus tequilensis and Calocybe indica in MDA-MB-231 human breast cancer cells: targeting p53 for anticancer therapy

Authors Gurunathan S, Park JH, Han JW, Kim JH

Received 4 March 2015

Accepted for publication 19 April 2015

Published 29 June 2015 Volume 2015:10(1) Pages 4203—4223

DOI https://doi.org/10.2147/IJN.S83953

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 2

Editor who approved publication: Dr Thomas J Webster

Sangiliyandi Gurunathan, Jung Hyun Park, Jae Woong Han, Jin-Hoi Kim

Department of Animal Biotechnology, Konkuk University, Seoul, Republic of Korea

Background:
Recently, the use of nanotechnology has been expanding very rapidly in diverse areas of research, such as consumer products, energy, materials, and medicine. This is especially true in the area of nanomedicine, due to physicochemical properties, such as mechanical, chemical, magnetic, optical, and electrical properties, compared with bulk materials. The first goal of this study was to produce silver nanoparticles (AgNPs) using two different biological resources as reducing agents, Bacillus tequilensis and Calocybe indica. The second goal was to investigate the apoptotic potential of the as-prepared AgNPs in breast cancer cells. The final goal was to investigate the role of p53 in the cellular response elicited by AgNPs.
Methods: The synthesis and characterization of AgNPs were assessed by various analytical techniques, including ultraviolet-visible (UV-vis) spectroscopy, X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, dynamic light scattering (DLS), and transmission electron microscopy (TEM). The apoptotic efficiency of AgNPs was confirmed using a series of assays, including cell viability, leakage of lactate dehydrogenase (LDH), production of reactive oxygen species (ROS), DNA fragmentation, mitochondrial membrane potential, and Western blot.
Results: The absorption spectrum of the yellow AgNPs showed the presence of nanoparticles. XRD and FTIR spectroscopy results confirmed the crystal structure and biomolecules involved in the synthesis of AgNPs. The AgNPs derived from bacteria and fungi showed distinguishable shapes, with an average size of 20 nm. Cell viability assays suggested a dose-dependent toxic effect of AgNPs, which was confirmed by leakage of LDH, activation of ROS, and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-positive cells in MDA-MB-231 breast cancer cells. Western blot analyses revealed that AgNPs induce cellular apoptosis via activation of p53, p-Erk1/2, and caspase-3 signaling, and downregulation of Bcl-2. Cells pretreated with pifithrin-alpha were protected from p53-mediated AgNPs-induced toxicity.
Conclusion: We have demonstrated a simple approach for the synthesis of AgNPs using the novel strains B. tequilensis and C. indica, as well as their mechanism of cell death in a p53-dependent manner in MDA-MB-231 human breast cancer cells. The present findings could provide insight for the future development of a suitable anticancer drug, which may lead to the development of novel nanotherapeutic molecules for the treatment of cancers.

Keywords:
apoptosis, UV-vis spectroscopy, X-ray diffraction, ROS generation

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]

 

Other articles by this author:

Novel biomolecule lycopene-reduced graphene oxide-silver nanoparticle enhances apoptotic potential of trichostatin A in human ovarian cancer cells (SKOV3)

Zhang XF, Huang FH, Zhang GL, Bai DP, Massimo DF, Huang YF, Gurunathan S

International Journal of Nanomedicine 2017, 12:7551-7575

Published Date: 13 October 2017

Zinc oxide nanoparticles induce apoptosis and autophagy in human ovarian cancer cells

Bai D, Zhang X, Zhang G, Huang Y, Gurunathan S

International Journal of Nanomedicine 2017, 12:6521-6535

Published Date: 5 September 2017

Silver nanoparticles cause complications in pregnant mice

Zhang XF, Park JH, Choi YJ, Kang MH, Gurunathan S, Kim JH

International Journal of Nanomedicine 2015, 10:7057-7071

Published Date: 13 November 2015

Effects of silver nanoparticles on neonatal testis development in mice

Zhang XF, Gurunathan S, Kim JH,

International Journal of Nanomedicine 2015, 10:6243-6256

Published Date: 5 October 2015

Reduced graphene oxide–silver nanoparticle nanocomposite: a potential anticancer nanotherapy

Gurunathan S, Han JW, Park JH, Kim E, Choi YJ, Kwon DN, Kim JH

International Journal of Nanomedicine 2015, 10:6257-6276

Published Date: 5 October 2015

Differential nanoreprotoxicity of silver nanoparticles in male somatic cells and spermatogonial stem cells

Zhang XF, Choi YJ, Han JW, Kim ES, Park JH, Gurunathan S, Kim JH

International Journal of Nanomedicine 2015, 10:1335-1357

Published Date: 16 February 2015

An in vitro evaluation of graphene oxide reduced by Ganoderma spp. in human breast cancer cells (MDA-MB-231)

Gurunathan S, Han J, Park JH, Kim JH

International Journal of Nanomedicine 2014, 9:1783-1797

Published Date: 8 April 2014

Ginkgo biloba: a natural reducing agent for the synthesis of cytocompatible graphene

Gurunathan S, Han JW, Park JH, Eppakayala V, Kim JH

International Journal of Nanomedicine 2014, 9:363-377

Published Date: 7 January 2014

Green chemistry approach for the synthesis of biocompatible graphene

Gurunathan S, Han JW, Kim JH

International Journal of Nanomedicine 2013, 8:2719-2732

Published Date: 31 July 2013

Green synthesis of graphene and its cytotoxic effects in human breast cancer cells

Gurunathan S, Han JW, Eppakayala V, Kim JH

International Journal of Nanomedicine 2013, 8:1015-1027

Published Date: 10 March 2013

Oxidative stress-mediated antibacterial activity of graphene oxide and reduced graphene oxide in Pseudomonas aeruginosa

Gurunathan S, Han JW, Dayem AA, Eppakayala V, Kim JH

International Journal of Nanomedicine 2012, 7:5901-5914

Published Date: 30 November 2012

Antitumor activity of silver nanoparticles in Dalton’s lymphoma ascites tumor model

Muthu Irulappan Sriram, Selvaraj Barath Mani Kanth, Kalimuthu Kalishwaralal, et al

International Journal of Nanomedicine 2010, 5:753-762

Published Date: 24 September 2010

Readers of this article also read:

Monitoring cancer stem cells: insights into clinical oncology

Lin SC, Xu YC, Gan ZH, Han K, Hu HY, Yao Y, Huang MZ, Min DL

OncoTargets and Therapy 2016, 9:731-740

Published Date: 11 February 2016

Effects of silver nanoparticles on neonatal testis development in mice

Zhang XF, Gurunathan S, Kim JH,

International Journal of Nanomedicine 2015, 10:6243-6256

Published Date: 5 October 2015

Reduced graphene oxide–silver nanoparticle nanocomposite: a potential anticancer nanotherapy

Gurunathan S, Han JW, Park JH, Kim E, Choi YJ, Kwon DN, Kim JH

International Journal of Nanomedicine 2015, 10:6257-6276

Published Date: 5 October 2015

Increased serum levels of interleukin-10 predict poor prognosis in extranodal natural killer/T-cell lymphoma patients receiving asparaginase-based chemotherapy

Wang H, Wang L, Wuxiao ZJ, Huang HQ, Jiang WQ, Li ZM, Lu Y, Xia ZJ

OncoTargets and Therapy 2015, 8:2589-2599

Published Date: 14 September 2015

Companion diagnostics and molecular imaging-enhanced approaches for oncology clinical trials

Van Heertum RL, Scarimbolo R, Ford R, Berdougo E, O’Neal M

Drug Design, Development and Therapy 2015, 9:5215-5223

Published Date: 11 September 2015

CaCO3/CaIP6 composite nanoparticles effectively deliver AKT1 small interfering RNA to inhibit human breast cancer growth

Zhou H, Wei J, Dai Q, Wang L, Luo J, Cheang T, Wang S

International Journal of Nanomedicine 2015, 10:4255-4266

Published Date: 1 July 2015

Tracking the 2015 Gastrointestinal Cancers Symposium: bridging cancer biology to clinical gastrointestinal oncology

Aprile G, Leone F, Giampieri R, Casagrande M, Marino D, Faloppi L, Cascinu S, Fasola G, Scartozzi M

OncoTargets and Therapy 2015, 8:1149-1156

Published Date: 22 May 2015

Multidisciplinary care in pediatric oncology

Cantrell MA, Ruble K

Journal of Multidisciplinary Healthcare 2011, 4:171-181

Published Date: 30 May 2011