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Co-Occurrence of the blaKPC-2 and Mcr-3.3 Gene in Aeromonas caviae SCAc2001 Isolated from Patients with Diarrheal Disease

Authors Tang L, Huang J, She J, Zhao K, Zhou Y

Received 10 January 2020

Accepted for publication 23 April 2020

Published 25 May 2020 Volume 2020:13 Pages 1527—1536

DOI https://doi.org/10.2147/IDR.S245553

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Dr Eric Nulens


Lingtong Tang,1,2,* Jianglian Huang,3,* Junping She,1 Kelei Zhao,4 Yingshun Zhou1

1Department of Pathogenic Biology, School of Basic Medicine, Southwest Medical University, Luzhou 646000, Sichuan, People’s Republic of China; 2Department of Clinical Laboratory, People’s Hospital of Gao County, Yibing 644000, Sichuan, People’s Republic of China; 3Department of Clinical Laboratory, The Second Affiliated Hospital of Xiamen Medical College, Xiaman 361600, People’s Republic of China; 4Antibiotics Research and Re-Evaluation Key Laboratory of Sichuan Province, Sichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu 610052, Sichuan, People’s Republic of China

*These authors contributed equally to this work

Correspondence: Yingshun Zhou
Department of Pathogenic Biology, School of Basic Medicine, Southwest Medical University, Luzhou, Sichuan 646000, People’s Republic of China, No. 319, Zhongshan Road, Tel +86-0830-3160073
Email yingshunzhou@swmu.edu.cn
Kelei Zhao
Antibiotics Research and Re-Evaluation Key Laboratory of Sichuan Province, Sichuan Industrial Institute of Antibiotics, No. 168, Huaguan Road, Chengdu 610052, Sichuan, People’s Republic of China
Tel +86– 028– 84216035
Email zhaokelei@cdu.edu.cn

Purpose: To characterize the genetic feature of a multi-drug-resistant Aeromonas caviae strain isolated from the diarrhea sample of a 45-year-old male patient with acute diarrhea.
Materials and Methods: Whole-genome of the A. caviae strain SCAc2001 was sequenced via the Illumina system, followed by a series of bioinformatic analyses to describe the genetic feature.
Results: The genome sequence of A. caviae SCAc2001 was assembled into 340 scaffolds (305 of them were > 1000 bp in length and 4,487,370 bp in total) with an average G+C content of 61.09%. Phylogenetic analysis showed that the A. caviae SCAc2001 strain was highly similar to the A. caviae strain R25-2 and T25-39. Resistome analysis identified that A. caviae SCAc2001 carried 13 antimicrobial resistance genes, including β-lactams (blaKPC, blaCTX-M-14, blaTEM-1, blaOXA-10, blaOXA-427, blaVEB-3 and blaMOX-6), aminoglycosides (aadA1), fluoroquinolones (aac(6ʹ)-Ib-cr), phenicol resistance (catB3), sulfonamide (sul1), trimethoprim (dfrA5) and colistin resistance (mcr-3.3).And also, A. caviae ScAc2001 carried 54 putative virulence genes including the type IV pilus, fimbria, flagellarthe, and hemolysin A encoding genes, and 12 pathogen–host interactions (PHI) genes. There were also four genomic islands and eight prophages in the genome of A. caviae ScAc2001. In addition, A. caviae SCAc2001 also carried three secondary metabolism products coding clusters including nonribosomal peptide synthetases (nrps), hserlactone and bacteriocin.
Conclusion: A. caviae ScAc2001 carries many resistance genes, a variety of virulence factors, PHI genes and four genomic islands and eight prophages, which poses a severe threat to infectious diseases control strategies, diagnosis methods and clinical treatment.

Keywords: Aeromonas caviae, blaKPC-2, mcr-3.3, virulence factors, secondary metabolism products
 

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