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Clinical significance of TIPE expression in gastric carcinoma

Authors Hu R, Qiu X, Hong S, Meng L, Hong X, Qiu J, Yang J, Zhuang G, Liu Z

Received 16 November 2015

Accepted for publication 22 May 2016

Published 25 July 2016 Volume 2016:9 Pages 4473—4481

DOI https://doi.org/10.2147/OTT.S100593

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Colin Mak

Peer reviewer comments 2

Editor who approved publication: Professor Min Li


Ruyi Hu,1,* Xingfeng Qiu,1,2,* Shifu Hong,1 Luxi Meng,1 Xinya Hong,3 Jinhua Qiu,1 Jingjing Yang,1 Guohong Zhuang,1,* Zhongchen Liu1,4

1Organ Transplantation Institute, Anti-Cancer Research Center, Medical College, Xiamen University, Fujian, People’s Republic of China; 2Division of Gastroenterology Surgery, Zhongshan Hospital, Gastroenterology Institute of Xiamen University, Gastroenterology Center of Xiamen, Fujian, People’s Republic of China; 3Fujian Medical University, Fujian, People’s Republic of China; 4Department of General Surgery, The Tenth People’s Hospital of Tongji University, Shanghai, People’s Republic of China

*These authors contributed equally to this work

Background: TNFAIP8, also known as TIPE, is a suppressor of apoptosis. High expression of both TIPE mRNA and protein has been detected in various cancer cell lines and clinical specimens compared to healthy tissues. Many reports have shown that there is a strong correlation between TIPE overexpression and cancer progression and poor prognosis in human solid cancers.
Methods: To illustrate the functional and clinical significance of TIPE in gastric cancer, we used reverse transcription polymerase chain reaction, quantitative real-time polymerase chain reaction, and immunohistochemistry to measure TIPE expression in clinical gastric specimens. Then, TIPE expression was knocked down by using shRNA and anti-DR5ScFv, to examine different expressions of TIPE in BGC823 cell lines, while cell proliferation and apoptosis were induced.
Results: We found that there was a strong correlation between TIPE expression and TNM stage (P=0.044), tumor depth (P=0.016), lymph node metastasis (P=0.026), and distant metastasis (P=0.045). No significant correlation was found between TIPE expression with the patients’ age (P=0.062) or sex (P=0.459). Anti-DR5ScFv induced TIPE depletion both in vitro and in vivo and resulted in apoptosis and suppression of proliferation.
Conclusion: Our results suggested that TIPE expression was associated with gastric cancer progression, and most importantly, suppressing TIPE expression might be an effective therapeutic strategy.

Keywords: TNFAIP8, gastric cancer, shRNA, anti-DR5ScFv, metastasis, apoptosis

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