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Clinical Significance of Has_circ_0060937 in Bone Metastasis of NSCLC

Authors Zhang J, Mao W, Chen Z, Gu H, Lian C

Received 22 September 2020

Accepted for publication 19 October 2020

Published 12 November 2020 Volume 2020:13 Pages 1115—1121


Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Dr Scott Fraser

Jiangnan Zhang,1 Wenwei Mao,2 Zhe Chen,2 Haiting Gu,2 Chan Lian2

1Orthopedics, The First People’s Hospital of Wenling, Wenling, Zhejiang, People’s Republic of China; 2Department of Respiratory and Critical Care Medicine, The First People’s Hospital of Wenling, Wenling, Zhejiang, People’s Republic of China

Correspondence: Chan Lian
Department of Respiratory and Critical Care Medicine, The First People’s Hospital of Wenling, Wenling 317500 Zhejiang, People’s Republic of China
Tel +86-15868658983

Background: Bone metastasis can induce multiple types of bone diseases which reduce the non-small-cell lung cancer (NSCLC) patient’s quality-of-life. Due to the difficulty of finding bone metastases and lack of effective early diagnosis, it is easy to miss the best treatment. Therefore, the study of serum tumor biomarkers is of great significance in the diagnosis of NSCLC bone metastasis.
Methods: The qRT-PCR assay was performed to assess has_circ_0060937 expression in 100 NSCLC patients. Furthermore, the small interfering RNAs si-has_circ_0060937 or si-NC were transfected into NSCLC bone metastasis cells. CCK8 assay was exercised to detect cell proliferation, and cell invasion assays were used to detect cell invasion in NSCLC bone metastasis cells.
Results: In this study, we firstly found that the expression of has_circ_0060937 in boneless metastasis NSCLC tissues and bone metastasis NSCLC tissues was significantly increased compared to normal tissues, and the expression of has_circ_0060937 was highest in bone metastasis. Expression of serum has_circ_0060937 in bone metastasis group from Grade I to Grade III NSCLC was drastically higher than boneless metastasis group or healthy group. In the Grade I to Grade III bone metastasis group, the expression of serum has_circ_0060937 gradually boosted with the increase of bone metastasis grades. Additionally, knockdown of has_circ_0060937 inhibited cell proliferation and cell invasion in NSCLC bone metastasis cell line.
Conclusion: The results suggestthat has_circ_0060937 is closely associated with bone metastasis in NSCLC, and the circRNAs we inspected may be a potential biomarker of bone metastasis in NSCLC.

Keywords: circRNA, has_circ_0060937, bone metastasis, NSCLC

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