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Clinical Performance of BD Kiestra InoqulA Automated System in a Chinese Tertiary Hospital

Authors Yue P, Zhou M, Zhang L, Yang Q, Song H, Xu Z, Zhang G, Xie X, Xu Y

Received 8 January 2020

Accepted for publication 10 March 2020

Published 1 April 2020 Volume 2020:13 Pages 941—947

DOI https://doi.org/10.2147/IDR.S245173

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Dr Sahil Khanna


Pinli Yue,1,* Menglan Zhou,1– 3,* Lintao Zhang,1,* Qiwen Yang,1 Hongmei Song,1 Zhipeng Xu,1 Ge Zhang,1 Xiuli Xie,1 Yingchun Xu1

1Department of Clinical Laboratory, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, People’s Republic of China; 2Graduate School, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, People’s Republic of China; 3Beijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, People’s Republic of China

*These authors contributed equally to this work

Correspondence: Qiwen Yang; Yingchun Xu Email yangqiwen81@vip.163.com; xycpumch@139.com

Background: The aim of this study was to evaluate the clinical performance of the BD Kiestra InoqulA automated specimen processing system with commonly encountered clinical microbiology specimens.
Materials and Methods: Four types of clinical specimens (sputum, urine, normally sterile body fluids, and feces) were inoculated onto relevant agar plates using a manual method and the BD Kiestra automated system. The number of isolated pathogen species, number of isolated single colonies and uniformity of plate streaking were calculated and compared between two methods.
Results: Significantly more isolated colonies were observed on plates inoculated by InoqulA for all specimen types and media with the exception of sputum specimens inoculated onto chocolate agar with vancomycin (P =0.076) and urine onto China blue agar (P =0.856). The quality of plate streaking was also better with InoqulA for all specimen types and media with the exception of urine specimens (P =1.000) and sterile body fluids (P =0.56) inoculated onto China blue agar.
Conclusion: This is the first evaluation study of InoqulA with 4 types of clinical specimens in China. It focused on the effect of streaking plates automatically with the magnetic bead. Inoculation of clinical specimens with the BD Kiestra InoqulA system is superior to the manual method for recovery of single colonies and the overall quality of semi-quantitative plate streaking.

Keywords: BD Kiestra, InoqulA, microbiology automation

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