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CK19 Promotes Ovarian Cancer Development by Impacting on Wnt/β-Catenin Pathway

Authors Lu Q, Qu H, Lou T, Liu C, Zhang Z

Received 18 December 2019

Accepted for publication 12 March 2020

Published 24 March 2020 Volume 2020:13 Pages 2421—2431

DOI https://doi.org/10.2147/OTT.S242778

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 2

Editor who approved publication: Dr Carlos E Vigil


Qi Lu, Hong Qu, Tong Lou, Chongdong Liu, Zhenyu Zhang

Department of Obstetrics and Gynecology, Chao-yang Hospital, Capital Medical University, Beijing, People’s Republic of China

Correspondence: Zhenyu Zhang; Chongdong Liu
Department of Obstetrics and Gynecology, Beijing Chao-yang Hospital, Capital Medical University, 8 Gongtinanlu, Chaoyang District, Beijing 100020, People’s Republic of China
Tel +86 13801237287
Fax +86 10 85231507
Email zhenyuzhang2000@163.com; 123630006@qq.com

Background: Epithelial ovarian cancer (EOC) is the most common type of ovarian cancer and is the most lethal gynecologic malignancy. Cytokeratin 19 (CK19) is a small type I cytokeratin. The aim of this study is to explore the functional role of CK19 and its underlying mechanism in EOC.
Methods: The expression levels of CK19 in EOC tissues were identified by Western blotting and RT-PCR assay. Transwell assay and CCK-8 proliferation assay were used to assess the invasion, migration and proliferation abilities of overexpressed or knockdown CK19 of ovarian cancer cells. We also detected the related genes of Wnt/β-catenin signal pathway, including β-catenin, TCF7, LEF1, c-MYC and cyclin D1 in the transfected ovarian cancer cells by Western blotting and RT-PCR assay.
Results: The results demonstrated that CK19 was upregulated in EOC tissue. CK19 was verified to promote the invasion, proliferation and migration of ovarian cancer cells. Additionally, CK19 activates the Wnt/β-catenin signaling pathway by upregulated β-catenin, TCF7, LEF1, c-MYC and cyclin D1.
Conclusions: In summary, this is the first study to investigate the role of CK19 in EOC. These findings provide a potential new therapeutic target for the clinical diagnosis and treatment of ovarian cancer.

Keywords: CK19, Wnt/β-catenin, ovarian cancer, SKOV3

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