Circular RNA FOXO3 Suppresses Bladder Cancer Progression and Metastasis by Regulating MiR-9-5p/TGFBR2
Authors Li Y, Qiao L, Zang Y, Ni W, Xu Z
Received 30 March 2020
Accepted for publication 5 June 2020
Published 25 June 2020 Volume 2020:12 Pages 5049—5056
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 2
Editor who approved publication: Professor Harikrishna Nakshatri
Yongxiang Li,1,2 Liang Qiao,2 Yuanwei Zang,1 Wenjun Ni,3 Zhonghua Xu1
1Department of Urology, Qilu Hospital of Shandong University, Jinan 250012, People’s Republic of China; 2Department of Urology, Weifang People’s Hospital, Weifang 261000, People’s Republic of China; 3Department of Urology, Zhuhai People’s Hospital (Zhuhai Hospital Affiliated with Jinan University), Zhuhai, People’s Republic of China
Correspondence: Zhonghua Xu
Department of Urology, Qilu Hospital of Shandong University, Jinan 250012, People’s Republic of China
Background: Increasing evidence indicates that the dysregulation of circular RNAs (circRNAs) plays important roles in tumor progressions.
Methods: In this study, we first analyzed circ-FOXO3 level in bladder cancers (BCs), and then BC cell lines were transfected with circ-FOXO3 expression vector, and cell proliferation, migration, and invasion abilities were analyzed. We also used bioinformatics tools to predict potential-binding miRNAs for circ-FOXO3, and luciferase reporter assay was used for the verification of binding miRNAs. For the further study, we analyzed potential downstream-binding mRNA for miRNA, and cell proliferation, migration and invasion abilities of it were also studied.
Results: We found that circ-FOXO3 was significantly down-regulated in bladder cancer (BC) tissues compared to normal bladder tissues. We also found that circ-FOXO3 overexpression inhibited cell proliferation, migration and invasion in BC cell lines. Moreover, we demonstrated that TGFBR2 was regulated by circ-FOXO3 through sponging miR-9-5p, the knockdown of TGFBR2 or the overexpression of miR-9-5p all related to the increased BC cell proliferation, migration, and invasion.
Discussion: In summary, our data showed that circ-FOXO3 was significantly down-regulated in bladder cancers. circ-FOXO3 overexpression inhibits BC cell progression and metastasis. Furthermore, circ-FOXO3 regulates TGFBR2 expression through sponging miR-9-5p in BC cell lines.
Keywords: bladder cancer, circ-FOXO3, miR-9-5p, TGFBR2
This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.Download Article [PDF] View Full Text [HTML][Machine readable]