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Circular RNA CircPRMT5 Accelerates Proliferation and Invasion of Papillary Thyroid Cancer Through Regulation of miR-30c/E2F3 Axis

Authors Xue C, Cheng Y, Wu J, Ke K, Miao C, Chen E, Zhang L

Received 11 February 2020

Accepted for publication 21 April 2020

Published 11 May 2020 Volume 2020:12 Pages 3285—3291


Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Dr Chien-Feng Li

Cheng Xue,1 Yi Cheng,1 Jinyou Wu,1 Kongliang Ke,2 Chundi Miao,2 Enfu Chen,1 Luqing Zhang2

1Department of Endocrinology, Wenling First People’s Hospital, Wenling 317500, People’s Republic of China; 2Department of Anorectal Surgery, Ningbo Hangzhou Bay Hospital, Ningbo 315000, People’s Republic of China

Correspondence: Luqing Zhang
Department of Anorectal Surgery, Ningbo Hangzhou Bay Hospital, 1155 Binhai 2nd Road, Hangzhouwan New District, Ningbo 315000, People’s Republic of China

Background: The role of circular RNA (circRNA) in papillary thyroid cancer (PTC) is largely unknown. This study aims to determine the function and mechanism of circPRMT5 in the regulation of PTC development.
Methods: PTC tissues and cell lines were used to determine circPRMT5 expression via quantitative real-time polymerase chain reaction. Small interfering RNA (siRNA) was utilized to knock down circPRMT5. Proliferation was analyzed through CCK8 and colony formation assays. Transwell assay was performed to determine cell migration and invasion. Luciferase assay and RIP assay were carried out to analyze the interaction between circPRMT5 and miR-30c.
Results: CircPRMT5 expression was upregulated in PTC tissues and cell lines. And circPRMT5 level was positively linked with advanced stage and lymph node metastasis. CircPRMT5 knockdown inhibited proliferation, migration and invasion while inducing apoptosis. CircPRMT5 worked as a competing endogenous RNA for miR-30c. By inhibiting miR-30c, circPRMT5 promoted the expression of E2F3.
Conclusion: Our findings demonstrate that circPRMT5 acts as an oncogenic circRNA to promote PTC progression via regulating miR-30c/E2F3 axis.

Keywords: circRNA, papillary thyroid cancer, circPRMT5, miR-30c, E2F3

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