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CircSKA3 Downregulates miR-1 Through Methylation in Glioblastoma to Promote Cancer Cell Proliferation

Authors Zhou M, Li H, Chen K, Ding W, Yang C, Wang X

Received 27 August 2020

Accepted for publication 7 November 2020

Published 19 January 2021 Volume 2021:13 Pages 509—514

DOI https://doi.org/10.2147/CMAR.S279097

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Dr Seema Singh


Meng Zhou,1 Huan Li,2 Ke’en Chen,1 Weilong Ding,1 Chengyou Yang,1 Xiangyu Wang1

1Department of Neurosurgery, The First Affiliated Hospital of Jinan University, Guangzhou City, Guangdong Province 510630, People’s Republic of China; 2Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, People’s Republic of China

Correspondence: Xiangyu Wang
Department of Neurosurgery, The First Affiliated Hospital of Jinan University, No. 613 West Huangpu Avenue, Tianhe District, Guangzhou City, Guangdong Province 510630, People’s Republic of China
Email xiangyuwangtianhe@163.com

Background: Circular RNA circSKA3 plays an oncogenic role in breast cancer, while its role in glioblastoma (GBM) is unknown. This study aimed to explore the role of circSKA3 in GBM.
Methods: Differential expression of circSKA3 and miR-1 in GBM and adjacent non-cancer tissue samples were analyzed by RT-qPCR. GBM cells were transfected with circSKA3 expression vector or miR-1 mimic, followed by RT-qPCR to explore the potential crosstalk between them. Methylation-specific PCR (MSP) was carried out to assess the role of circSKA3 in regulating the methylation of miR-1 gene. The role of circSKA3 and miR-1 in regulating GBM cell proliferation was analyzed by CCK-8 assay.
Results: We found that circSKA3 was upregulated in GBM and inversely correlated with miR-1 across GBM tissues. High expression levels of circSKA3 and low expression levels of miR-1 were significantly correlated with the poor survival of GBM patients. In GBM cells, overexpression of circSKA3 increased the methylation of miR-1 gene and decreased the expression of miR-1. CCK-8 assay showed that overexpression of circSKA3 reduced the inhibitory effects of miR-1 on cell proliferation.
Conclusion: Therefore, circSKA3 may downregulate miR-1 through methylation in GBM to promote cancer cell proliferation.

Keywords: glioblastoma, circSKA3, miR-1, methylation

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