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CircHIPK3 Facilitates the G2/M Transition in Prostate Cancer Cells by Sponging miR-338-3p

Authors Liu F, Fan Y, Ou L, Li T, Fan J, Duan L, Yang J, Luo C, Wu X

Received 15 December 2019

Accepted for publication 1 May 2020

Published 22 May 2020 Volume 2020:13 Pages 4545—4558

DOI https://doi.org/10.2147/OTT.S242482

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 2

Editor who approved publication: Dr Tohru Yamada


Fengchun Liu,1 Yanru Fan,1 Liping Ou,1 Ting Li,1 Jiaxin Fan,1 Limei Duan,1 Jinxiao Yang,1 Chunli Luo,1 Xiaohou Wu2

1Department of Laboratory Diagnosis, Chongqing Medical University, Yuzhong, Chongqing 408000, People’s Republic of China; 2Department of Urology, The First Affiliated Hospital of Chongqing Medical University, Yuzhong, Chongqing 408000, People’s Republic of China

Correspondence: Xiaohou Wu
Department of Urology, The First Affiliated Hospital of Chongqing Medical University, No. 1 Medical College Road, Yuzhong, Chongqing 408000, People’s Republic of China
Email wuxiaohou163@163.com
Chunli Luo
Department of Laboratory Diagnosis, Chongqing Medical University, No. 1 Medical College Road, Yuzhong, Chongqing 408000, People’s Republic of China
Email luochunli79@126.com

Background: Circular RNAs (circRNAs) play a crucial role in gene expression regulation. CircHIPK3 is a circRNA derived from Exon 2 of HIPK3 gene and its role in prostate cancer (PCa) is still unclear.
Methods: CCK8 assays, flow cytometry and colony formation assays were performed to assess the effects of circHIPK3 in PCa cells. Bioinformatics analysis, RNA pull-down assay, RNA immunoprecipitation assay (RIP), and luciferase activity assay were performed to dissect the mechanism underlying circHIPK3-mediated G2/M transition in PCa cells.
Results: CircHIPK3 expression was upregulated in PCa cells and prostate cancer tissues. Overexpression of circHIPK3 or circHIPK3 silencing altered PCa viability, proliferation and apoptosis in vitro. CircHIPK3 could sponge miR-338-3p and inhibit its activity, resulting in increased expression of Cdc25B and Cdc2 in vitro.
Conclusion: CircHIPK3 promotes G2/M transition and induces PCa cell proliferation by sponging miR-338-3p and increasing the expression of Cdc25B and Cdc2. CircHIPK3 may play an oncogenic role in PCa.

Keywords: prostate cancer, circHIPK3, miR-338-3p, Cdc25B, G2/M transition

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