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CircCDYL Serves as a New Biomarker in Mantle Cell Lymphoma and Promotes Cell Proliferation

Authors Mei M, Wang Y, Wang Q, Liu Y, Song W, Zhang M

Received 23 September 2019

Accepted for publication 15 November 2019

Published 3 December 2019 Volume 2019:11 Pages 10215—10221

DOI https://doi.org/10.2147/CMAR.S232075

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 2

Editor who approved publication: Dr Eileen O'Reilly


Mei Mei,1,2 Yingjun Wang,1 Qilong Wang,1 Yueyao Liu,3 Wenting Song,1 Mingzhi Zhang1

1Department of Oncology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, People’s Republic of China; 2The Academy of Medical Sciences, Zhengzhou University, Zhengzhou, People’s Republic of China; 3Department of Pediatrics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, People’s Republic of China

Correspondence: Mingzhi Zhang
Department of Oncology, The First Affiliated Hospital of Zhengzhou University, No.1 East Jianshe Road, Zhengzhou, People’s Republic of China
Tel +86 138 3856 5629
Fax +86 371 6629 5561
Email mingzhi_zhang1@163.com

Introduction: Mantle cell lymphoma (MCL) is a rare subtype of B-cell lymphoma. Circular (circ) RNA is a member of the non-coding RNA family. However, clinical references to circRNAs in MCL are not clear.
Methods: In this study, we detected the expression level of circCDYL in the plasma of MCL patients compared to healthy donors by the quantitative reverse transcription polymerase chain reaction. The diagnostic value of circCDYL was determined using a receiver operating characteristic (ROC) curve. We constructed a circCDYL short hairpin RNA plasmid and infected the MCL cell line, Z138, to detect its effect on cell proliferation.
Results: CircCDYL was high expressed in the plasma of MCL patients. The ROC curve showed that circCDYL had diagnostic value (area under the curve (AUC) = 0.856). Functionally, circCDYL knockdown inhibited MCL cell proliferation. We conducted bioinformatics analyses and identified a circCDYL-micro (mi)RNA–mRNA/long non-coding (lnc)RNA network, highlighted by five miRNAs (hsa-miR-129-5p, hsa-miR-3163, hsa-miR-4662a-5p, hsa-miR-101-3p, and hsa-miR-186-5p), three lncRNAs (MALAT1, NEAT1, and XIST), and five mRNAs (NOTCH1, FMR1, ABCB1, TWIST1, and VEGFA).
Conclusion: These findings indicate that circ-CDYL might serve as a potential diagnostic biomarker in clinical practice.

Keywords: circular RNA, biomarker, AUC, diagnosis, non-coding RNA
 

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