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Characterization of antibiotic resistance and virulence factors of Escherichia coli strains isolated from Iranian inpatients with urinary tract infections

Authors Haghighatpanah M, Mojtahedi A

Received 17 June 2019

Accepted for publication 24 August 2019

Published 3 September 2019 Volume 2019:12 Pages 2747—2754


Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 3

Editor who approved publication: Professor Suresh Antony

Maryam Haghighatpanah1, Ali Mojtahedi2

1Department of Microbiology, Islamic Azad University, Lahijan Branch, Guilan, Iran; 2Department of Microbiology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran

Correspondence: Ali Mojtahedi
Department of Microbiology, School of Medicine, Guilan University Campus, 7 th Km of Rasht-Tehran Highway, Rasht, Guilan, Iran
Tel +98 133 369 0884
Fax +98 133 369 0036

Background: Urinary tract infections (UTIs) are one of the most frequent human infectious diseases causing considerable amount of morbidity and mortality. The present study aimed to investigate the occurrence of antibiotics resistance and virulence genes among Escherichia coli strains isolated from UTIs in the north of Iran.
Methods: This cross-sectional study was performed at 5 teaching hospitals in Rasht in the north of Iran. Totally, 129 E. coli isolates were identified by standard microbiologic tests. Antimicrobial susceptibility pattern was determined using disk diffusion method. The presence of virulence genes was detected by PCR method.
Results: The results of antibiotic susceptibility showed that the highest resistance rates were to ampicillin (78.3%) followed by nalidixic acid (74.4%) and trimethoprim/sulfamethoxazole (69.8%). On the other hand, the highest susceptibility was toward nitrofurantoin (96.1%) and imipenem (92.2%). Further analysis revealed that the rate of ESBL-producing and multiple-drug resistant isolates was 51.2% and 84.5%, respectively. Molecular analysis revealed that traaT (87.6%) gene was the most prevalent virulence factors followed by fyuA (86%) and kpsmt (76%) genes. Also, fimH gene was the most frequently detected adhesion-associated gene with 74.4%.
Conclusion: In summary, our results showed a remarkable rate of drug resistance and heterogeneity for virulence factors among E. coli strains isolated from UTIs in the north of Iran. The emergence of such strains can be a predictive marker for their persistence in the hospital and consequently a significant threat for hospitalized patients.

Keywords: urinary tract infections, Escherichia coli, antibiotics resistance, ESBL, virulence factors

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