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Characterization of a Novel mcr-8.2-Bearing Plasmid in ST395 Klebsiella pneumoniae of Chicken Origin

Authors Yang X, Peng K, Zhang Y, Liu L, Li R

Received 3 April 2020

Accepted for publication 16 May 2020

Published 16 June 2020 Volume 2020:13 Pages 1781—1784

DOI https://doi.org/10.2147/IDR.S256544

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 3

Editor who approved publication: Professor Suresh Antony


Xiaorong Yang,1,* Kai Peng,2,3,* Yuxia Zhang,4 Li Liu,1 Ruichao Li2,3

1Center for Disease Control and Prevention of Sichuan Province, Chengdu, People’s Republic of China; 2Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, People’s Republic of China; 3Institute of Comparative Medicine, Yangzhou University, Yangzhou, People’s Republic of China; 4Institute of Qinghai-Tibet Plateau, Southwest Minzu University, Chengdu, People’s Republic of China

*These authors contributed equally to this work

Correspondence: Ruichao Li
College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu Province, People’s Republic of China
Email rchl88@yzu.edu.cn

Abstract: The emergence of mobile colistin resistance mcr genes undermines the efficacy of colistin as the last-resort drug for multi-drug resistance infections and constitutes a great public health concern. Plasmids play a critical role in the transmission of mcr genes among bacteria. One colistin-resistant Klebsiella pneumoniae strain of chicken origin was collected and analyzed by antimicrobial susceptibility testing, PCR, conjugation assay and S1-PFGE. Whole-genome sequencing (WGS) approach combining Illumina and MinION platforms was utilized to decipher the underlying colistin resistance mechanism and genetic context. A novel mcr-8.2-bearing plasmid p2019036D-mcr8-345kb with 345 655 bp in size encoding various resistance genes including floR, sul1, aadA16, aadA2, blaCTX-M-27, blaDHA-1, tet(D), dfrA12 and qnrB4 was identified responsible for the colistin resistance phenotype. Plasmid comparison has shown that the mcr-8.2-bearing plasmid differed from other reported plasmids positive for mcr-8.2 but shared the same core mcr-8.2-bearing conserved region. This study demonstrates the emergence of mcr-8.2-bearing K. pneumoniae of animal origin is a potential risk to humans.

Keywords: mcr-8.2, Klebsiella pneumoniae, plasmids, animal origin

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