Back to Journals » International Journal of Nanomedicine » Volume 7

Cell responses to two kinds of nanohydroxyapatite with different sizes and crystallinities

Authors Liu X, Zhao M, Lu J, Ma J, Wei J, Wei S

Received 9 November 2011

Accepted for publication 1 December 2011

Published 7 March 2012 Volume 2012:7 Pages 1239—1250


Review by Single-blind

Peer reviewer comments 4

Xiaochen Liu1, Minzhi Zhao1, Jingxiong Lu2, Jian Ma4, Jie Wei2, Shicheng Wei1,3
1Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, 2Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai, 3Department of Oral and Maxillofacial Surgery, School of Stomatology, Peking University, Beijing, 4Hospital of Stomatology, Tongji University, Shanghai, China

Introduction:Hydroxyapatite (HA) is the principal inorganic constituent of human bone. Due to its good biocompatibility and osteoconductivity, all kinds of HA particles were prepared by different methods. Numerous reports demonstrated that the properties of HA affected its biological effects.
Methods: Two kinds of nanohydroxyapatite with different sizes and crystallinities were obtained via a hydrothermal treatment method under different temperatures. It was found that at a temperature of 140°C, a rod-like crystal (n-HA1) with a diameter of 23 ± 5 nm, a length of 47 ± 14 nm, and crystallinity of 85% ± 5% was produced, while at a temperature of 80°C, a rod-like crystal (n-HA2) with a diameter of 16 ± 3 nm, a length of 40 ± 10 nm, and crystallinity of 65% ± 3% was produced. The influence of nanohydroxyapatite size and crystallinity on osteoblast viability was studied by MTT, scanning electron microscopy, and flow cytometry.
Results: n-HA1 gave a better biological response than n-HA2 in promoting cell growth and inhibiting cell apoptosis, and also exhibited much more active cell morphology. Alkaline phosphatase activity for both n-HA2 and n-HA1 was obviously higher than for the control, and no significant difference was found between n-HA1 and n-HA2. The same trend was observed on Western blotting for expression of type I collagen and osteopontin. In addition, it was found by transmission electron microscopy that large quantities of n-HA2 entered into the cell and damaged the cellular morphology. Release of tumor necrosis factor alpha from n-HA2 was markedly higher than from n-HA1, indicating that n-HA2 might trigger a severe inflammatory response.
Conclusion: This work indicates that not all nanohydroxyapatite should be considered a good biomaterial in future clinical applications.

Keywords: nanohydroxyapatite, osteoblast-like cells, cell viability, cell differentiation

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]


Readers of this article also read:

Fabrication of nanoadjuvant with poly-e-caprolactone (PCL) for developing a single-shot vaccine providing prolonged immunity

Prashant CK, Bhat M, Srivastava SK, Saxena A, Kumar M, Singh A, Samim M, Ahmad FJ, Dinda AK

International Journal of Nanomedicine 2014, 9:937-950

Published Date: 12 February 2014

Antimicrobial activity of metal oxide nanoparticles against Gram-positive and Gram-negative bacteria: a comparative study

Azam A, Ahmed AS, Oves M, Khan MS, Habib SS, Memic A

International Journal of Nanomedicine 2012, 7:6003-6009

Published Date: 5 December 2012

Development and characterization of a new oral dapsone nanoemulsion system: permeability and in silico bioavailability studies

Monteiro LM, Lione VF, do Carmo FA, do Amaral LH, da Silva JH, Nasciutti LE, Rodrigues CR, Castro HC, de Sousa VP, Cabral LM

International Journal of Nanomedicine 2012, 7:5175-5182

Published Date: 28 September 2012

A sandwich-type DNA electrochemical biosensor for hairpin-stem-loop structure based on multistep temperature-controlling method

Hong GL, Liu YH, Chen W, Weng SH, Liu QC, Liu AL, Zheng DX, Lin XH

International Journal of Nanomedicine 2012, 7:4953-4960

Published Date: 14 September 2012

Kaempferol nanoparticles achieve strong and selective inhibition of ovarian cancer cell viability

Luo H, Jiang B, Li B, Li Z, Jiang BH, Chen YC

International Journal of Nanomedicine 2012, 7:3951-3959

Published Date: 24 July 2012

Multifunctional targeting micelle nanocarriers with both imaging and therapeutic potential for bladder cancer

Lin TY, Zhang H, Luo J, Li Y, Gao T, Lara Jr PN, de Vere White R, Lam KS, Pan CX

International Journal of Nanomedicine 2012, 7:2793-2804

Published Date: 6 June 2012

Thermal and ultrasonic influence in the formation of nanometer scale hydroxyapatite bio-ceramic

Poinern GE, Brundavanam RK, Le XT, Djordjevic S, Prokic M, Fawcett D

International Journal of Nanomedicine 2011, 6:2083-2095

Published Date: 23 September 2011