Cell classification by moments and continuous wavelet transform methods

Qian Chen¹, Yuan Fan^1,2, Lalita Udpa², Virginia M Ayres¹

¹Electronic and Biological Nanostructures Laboratory, ²Nondestructive Evaluation Laboratory, College of Engineering, Michigan State University, East Lansing, MI, USA

Abstract: Image processing techniques are bringing new insights to biomedical research. The automatic recognition and classification of biomedical objects can enhance work efficiency while identifying new inter-relationships among biological features. In this work, a simple rule-based decision tree classifier is developed to classify typical features of mixed cell types investigated by atomic force microscopy (AFM). A combination of continuous wavelet transform (CWT) and moment-based features are extracted from the AFM data to represent that shape information of different cellular objects at multiple resolution levels. The features are shown to be invariant under operations of translation, rotation, and scaling. The features are then used in a simple rulebased classifier to discriminate between anucleate versus nucleate cell types or to distinguish cells from a fibrous environment such as a tissue scaffold or stint. Since each feature has clear physical meaning, the decision rule of this tree classifier is simple, which makes it very suitable for online processing. Experimental results on AFM data confirm that the performance of this classifier is robust and reliable.

Keywords: cell classification, atomic force microscopy, second moment, continuous wavelet transform