Bronchoscopic thermal vapor ablation in a canine model of emphysema

Stephanie A Tuck¹, Vanessa Lopes-Berkas², Sheree Beam³, Joseph C Anderson^1
1Uptake Medical Corp, Seattle, WA, ²American Preclinical Services, Coon Rapids, MN, ³Preclinical Pathology Consulting Services, Ham Lake, MN, USA

Abstract: Clinical studies indicate the potential of bronchoscopic thermal vapor ablation to result in clinically relevant improvements in severe chronic obstructive pulmonary disease patients with upper lobe-predominant emphysema. However, the mechanisms by which vapor ablation results in lung volume reduction are not fully known. This study determined the 3-month safety and efficacy of vapor ablation in a canine model of emphysema and described the histopathological changes in the lung. The cranial lobes of papain-exposed dogs were treated with a vapor dose of ten calories per gram of lung tissue (n = 8) or were sham treated (n = 3). Safety was monitored peri- and postoperatively for 3 months. Animals were then sacrificed, estimates of lung volume reduction performed, and the lungs processed for histology. Vapor ablation was associated with an average of 20% volume reduction of the treated lobes and an absence of serious adverse events. The amount of lobar volume reduction was correlated with the amount of fibrosis and atelectasis in the treated lobe. Bronchoscopic thermal vapor ablation at a dose of 10 cal/g results in lobar volume reduction associated with remodeling of the targeted tissue characterized by mature collagen formation in the absence of major adverse events.

Keywords: animal models, chronic obstructive pulmonary disease, bronchoscopy, lung volume reduction