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Bovine Meniscus Middle Zone Tissue: Measurement of Collagen Fibril Behavior During Compression

Authors Sizeland KH, Wells HC, Kirby NM, Hawley A, Mudie ST, Ryan TM, Haverkamp RG

Received 7 May 2020

Accepted for publication 9 July 2020

Published 27 July 2020 Volume 2020:15 Pages 5289—5298

DOI https://doi.org/10.2147/IJN.S261298

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 3

Editor who approved publication: Prof. Dr. Anderson Oliveira Lobo


Katie H Sizeland,1 Hannah C Wells,2 Nigel M Kirby,3 Adrian Hawley,3 Stephen T Mudie,3 Tim M Ryan,3 Richard G Haverkamp2

1Human Health, ANSTO, Lucas Heights, Sydney, NSW 2234, Australia; 2School of Food and Advanced Technology, Massey University, Palmerston North 4472, New Zealand; 3SAXS/WAXS Beamline, Australian Synchrotron, ANSTO, Clayton, Melbourne, VIC 3168, Australia

Correspondence: Richard G Haverkamp
School of Food and Advanced Technology, Massey University, Private Bag 1112, Palmerston North 4472, New Zealand
Tel +64 6 356 9099
Email r.haverkamp@massey.ac.nz

Background: Type I collagen is the major component of the extracellular matrix of the knee’s meniscus and plays a central role in that joint’s biomechanical properties. Repair and reconstruction of tissue damage often requires a scaffold to assist the body to rebuild. The middle zone of bovine meniscus is a material that may be useful for the preparation of extracellular matrix scaffolds.
Methods: Here, synchrotron-based small-angle X-ray scattering (SAXS) patterns of bovine meniscus were collected during unconfined compression. Collagen fibril orientation, D-spacing, compression distance and force were measured.
Results: The collagen fibrils in middle zone meniscal fibrocartilage become more highly oriented perpendicular to the direction of compression. The D-spacing also increases, from 65.0 to 66.3 nm with compression up to 0.43 MPa, representing a 1.8% elongation of collagen fibrils perpendicular to the compression.
Conclusion: The elasticity of the collagen fibrils under tension along their length when the meniscus is compressed, therefore, contributes to the overall elastic response of the meniscus only under loads that exceed those likely to be experienced physiologically.

Keywords: collagen, meniscus, compression, SAXS, scaffold

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