Back to Journals » Drug Design, Development and Therapy » Volume 14

Bone Marrow Mesenchymal Stem Cell-Derived Exosomes Improves Spinal Cord Function After Injury in Rats by Activating Autophagy

Authors Gu J, Jin ZS, Wang CM, Yan XF, Mao YQ, Chen S

Received 6 November 2019

Accepted for publication 18 March 2020

Published 29 April 2020 Volume 2020:14 Pages 1621—1631

DOI https://doi.org/10.2147/DDDT.S237502

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 3

Editor who approved publication: Dr Yan Zhu


Jun Gu, Zheng Shuai Jin, Chun Ming Wang, Xue Fei Yan, Yuan Qing Mao, Sheng Chen

The Affiliated Jiangsu Shengze Hospital of Nanjing Medical University, Suzhou 215228, People’s Republic of China

Correspondence: Sheng Chen Tel +8618013798189
Email rylnjmu@sina.com

Background: Spinal cord injury (SCI) is a global medical problem. The smallest membrane-bound nanovesicles, known as exosomes, have a role in complex intercellular communication systems and can be used directly as therapeutic agents by acting as important paracrine factors. Nevertheless, the use of exosomes derived from BMSCs (BMSC-Exos) to treat SCI has been less, and the specific mechanism has not yet been reported.
Methods: BMSC-Exos were characterized by TEM, NTA and Western blot. The effects of BMSC-Exos treatment were compared by SCI in vivo model and a series of in vitro experiments.
Results: BMSC-Exos were found to enhance the expression of autophagy-related proteins LC3IIB and Beclin-1 and enabled autophagosomes formation. After BMSC-Exos treatment, there was marked decline in the level of expression of proapoptotic protein cleaved caspase-3, while that of the antiapoptotic protein Bcl-2 was upregulated.
Conclusion: BMSC-Exos can attenuate neuronal apoptosis by promoting autophagy and promote the potential efficacy of functional behavior recovery in SCI rats. In summary, these findings expand the theoretical knowledge and forms a realistic route for the future treatment of SCI by BMSC-Exos.

Keywords: bone marrow mesenchymal stem cells, exosomes, spinal cord injury, apoptosis, autophagy

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]