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Biodistribution, Clearance And Morphological Alterations Of Intravenously Administered Iron Oxide Nanoparticles In Male Wistar Rats

Authors Gaharwar US, Meena R, Rajamani P

Received 14 July 2019

Accepted for publication 10 October 2019

Published 6 December 2019 Volume 2019:14 Pages 9677—9692

DOI https://doi.org/10.2147/IJN.S223142

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 2

Editor who approved publication: Dr Thomas Webster


Usha Singh Gaharwar, Ramovatar Meena, Paulraj Rajamani

School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, India

Correspondence: Paulraj Rajamani
School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, India
Tel +91 11 2670 4162
Email paulrajr@hotmail.com

Introduction: Nanoparticles are used worldwide because of their unique properties, with large-scale application in various fields, such as medicine, cosmetics and industries. In view of their widespread use, the potential adverse effects of nanoparticles have become a significant cause for concern, in terms of not only human health and safety but also the environment. The present investigation focused on establishing the bioaccumulation patterns and ultrastructural changes induced by retained iron oxide nanoparticles (IONPs) in various target organs of rats.
Methods: Twenty-four male Wistar rats were randomly divided into four groups. Experimental animals were intravenously administered different doses of IONPs (7.5 mg/kg, 15 mg/kg and 30 mg/kg) once in a week for 4 weeks. Urine and feces samples were collected on a daily basis to assess nanoparticle clearance and analyzed via atomic absorption spectroscopy (AAS). At the end of the experiment, rats were euthanized and different organs, including spleen, liver, kidney, lung, heart, testis and brain, were dissected. Bioaccumulation of iron in organs and ultrastructural changes induced by IONPs were determined.
Results: The maximal concentration of iron was detected in spleen and minimal concentration in the brain. The level of iron accumulation in organs was as follows: spleen>blood>liver>kidney>lung>heart>testis>brain. The excretion profile in urine revealed maximum excretion on the day following administration that was maintained until day 28, whereas the iron content in feces remained high during the first three days after injection. A similar pattern was observed throughout the duration of the experiment. Ultrastructural alterations were detected in spleen, kidney, lung, heart, testis, brain and liver, indicative of cellular damage induced by accumulating nanoparticles in these organs.
Conclusion: Intravenous administration of IONPs results in ultrastructural changes and dose-dependent bioaccumulation in different organs of rats.

Keywords: metal oxide nanoparticles, bioaccumulation, ultrastructural changes, toxicity, metabolic cages


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