Biocompatibility of the vital dye Acid Violet-17 on retinal pigment epithelial cells
Authors Tura A, Alt A, Lüke J, Grisanti S, Haritoglou C, Meyer CH, Nassar K, Lüke M
Received 3 February 2016
Accepted for publication 9 March 2016
Published 29 July 2016 Volume 2016:10 Pages 1435—1445
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Colin Mak
Peer reviewer comments 2
Editor who approved publication: Dr Scott Fraser
Ayşegül Tura,1 Aizhan Alt,1 Julia Lüke,1 Salvatore Grisanti,1 Christos Haritoglou,2 Carsten H Meyer,3 Khaled Nassar,1 Matthias Lüke1
On behalf of the International Chromovitrectomy Collaboration
1Department of Ophthalmology, University of Schleswig-Holstein, Lübeck, Germany; 2Department of Ophthalmology, Ludwig-Maximilians University, Munich, Germany; 3Department of Ophthalmology, Pallas Clinic, Aarau, Switzerland
Purpose: To examine the viability and differentiation of retinal pigment epithelial (RPE) cells after exposure to the vital dye Acid Violet-17 (AV-17).
Methods: Bovine RPE cells were incubated with AV-17 (0.0625–0.5 mg/mL) for 30 seconds or 5 minutes. Viability was determined by live/dead staining, cleaved CASP3 immunostainings, and MTT test. Actin cytoskeleton was visualized by Alexa 488-phalloidin. Immunocytochemistry was performed to determine the levels of ZO-1, CTNNB1, and KRT19.
Results: Exposure to AV-17 at the concentrations of 0.25–0.5 mg/mL resulted in a dose-dependent decrease in viability, the loss of ZO-1 from tight junctions, translocation of CTNNB1 into the cytoplasm and nucleus, disarrangement of the actin cytoskeleton, and a slight increase in KRT19.
Conclusion: AV-17 at a concentration <0.125 mg/mL is likely to be well tolerated by the RPE cells, whereas the concentrations from 0.25 mg/mL onward can reduce viability and induce dedifferentiation particularly after long-term exposure.
Keywords: Acid Violet-17, retinal pigment epithelial cells, biocompatibility, viability, differentiation
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