Binding fullerenol C60(OH)24 to dsDNA
Mariana Pinteala, Andrei Dascalu, Cezar Ungurenasu
Petru Poni Institute of Macromolecular Chemistry, Aleea Grigore Ghica, Iasi, Romania
Abstract: The first C60(OH)24-DNA complex and its fluorescence enhancement is reported. The enhanced fluorescence intensity of fullerenol C60(OH)24 is in proportion to the concentration of DNA in the range of 1 × 10-9 to 8 × 10-5 molL-1 and the detection limit was 1.3 ng mL-1. Fullerenol C60(OH)24 binds significantly to the phosphate backbone of native dsDNA and to base-pairs within the major groove of sodium salt of dsDNA.
Keywords: nanomedicine, fullerenol, DNA complexation, fluorescent probe
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