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Bacterial magnetosomes-based nanocarriers for co-delivery of cancer therapeutics in vitro

Authors Long R, Dai Q, Zhou X, Cai D, Hong Y , Wang SB, Liu Y

Received 27 July 2018

Accepted for publication 6 November 2018

Published 4 December 2018 Volume 2018:13 Pages 8269—8279

DOI https://doi.org/10.2147/IJN.S180503

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 3

Editor who approved publication: Dr Mian Wang



Rui-Min Long,1,2,* Qing-Lei Dai,1,* Xia Zhou,1,* Duan-Hua Cai,1 Ya-Zhen Hong,1–3 Shi-Bin Wang,1–4 Yuan-Gang Liu1–3

1College of Chemical Engineering, Huaqiao University, Xiamen 361021, China; 2Fujian Provincial Key Laboratory of Biochemical Technology, Huaqiao University, Xiamen 361021, China; 3Institute of Pharmaceutical Engineering, Huaqiao University, Xiamen 361021, China; 4Institute of Biomaterials and Tissue Engineering, Huaqiao University, Xiamen 361021, China

*These authors contributed equally to this work

Abstract: In recent times, co-delivery of therapeutics has emerged as a promising strategy for treating dreadful diseases such as cancer.
Materials and methods: In this study, we developed a novel nanocarrier based on bacterial magnetosomes (BMs) that co-loaded with siRNA and doxorubicin (DOX) using polyethyleneimine (PEI) as a cross-linker (BMs/DP/siRNA). The delivery efficiency of siRNA as well as the pH-responsive release of DOX, and synergistic efficacy of these therapeutics in vitro were systematically investigated.
Results: The structure of DOX–PEI (DP) conjugates that synthesized via hydrazone bond formation was confirmed by 1H nuclear magnetic resonance (NMR). The in vitro release experiments showed that the DP conjugate (DOX-loading efficiency – 5.77%±0.08%) exhibited the long-term release behavior. Furthermore, the optimal BMs/DP/siRNA particle size of 107.2 nm and the zeta potential value of 31.1±1.0 mV facilitated enhanced cellular internalization efficiency. Moreover, the agarose gel electrophoresis showed that the co-delivery system could protect siRNA from degradation in serum and RNase A. In addition, the cytotoxicity assay showed that BMs/DP/siRNA could achieve an excellent synergistic effect compared to that of siRNA delivery alone. The acridine orange (AO)/ethidium bromide (EB) double staining assay also showed that BMs/DP/siRNA complex could induce cells in a stage of late apoptosis and nanocomplex located in the proximity of the nucleus.
Conclusion: The combination of gene and chemotherapeutic drug using BMs is highly efficient, and the BMs/DP/siRNA would be a promising therapeutic strategy for the future therapeutics.

Keywords: bacterial magnetosomes, co-delivery, gene therapy, pH-responsive release

 

Introduction

Currently, the co-delivery of therapeutics that particularly includes the combination of nucleic acids and chemotherapeutic drugs has attracted enormous interest due to their enormous therapeutic efficacy over those delivered solely. In addition, this strategy offers numerous advantages such as the use of limited doses of cytotoxic agents resulting in the reduced side effects, delay in the development of cancer drug resistance, leading to augmented intracellular drug levels, and could deliver multiple therapeutic agents that act through different antiproliferative mechanisms.1,2 Therefore, an ideal carrier platform is required to protect nucleic acid from degradation and enable to control the release of the gene as well as chemotherapeutic agent, for synergistic therapeutic efficacy.

In recent years, considerable efforts have been put forward to construct the co-delivery systems. For example, the co-delivery of doxorubicin (DOX) and valproic acid could result in the internalization of latter into HepG2 cell line when co-delivered illustrating efficient combination therapy.3 Moreover, the nanocarriers including liposomes,4,5 polymers,69 and several inorganic materials such as mesoporous silica nanoparticles and layered double hydroxides1013 have been used for delivering genes and drugs.14 Among them, polyethyleneimine (PEI), a cationic polymer, has been widely used for gene delivery due to its advantages of highly positive charge and abundant amine groups.1517 In this framework, chemotherapeutic drug conjugates with the cationic polymer via pH-sensitive linker followed by binding with gene through electrostatic interactions, which can achieve an effective combinatorial delivery of nucleic acid and chemotherapeutic drug.18 The obtained delivery system can not only avoid enzyme degradation but also control the release rate of therapeutic drugs.

Bacterial magnetosomes (BMs) have emerged as a novel and efficient material due to their advantages of excellent biocompatibility and abundant bioactive sites in the membrane.19,20 BMs are membrane-covered nanocrystals of magnetic iron oxide or iron sulfide synthesized by magnetotactic bacteria.21 The advantageous properties of them make BMs a prior candidate for the delivery of proteins, chemotherapeutic drugs, and genes, among others.2224 Compared to synthetic magnetic nanoparticles, BMs offer various advantages, such as cost-effective, ease of synthesis, and surface modification, which make them more promising to become the nanocarrier for the co-delivery of therapeutics.25

Here, we designed an efficient co-delivery platform based on siRNA- and DOX-loaded BMs using PEI as a cross-linker (BMs/DP/siRNA) for cancer therapeutics. First, the bifunctional succinimidyl 6-hydrazinonicotinate acetone hydrazine (SANH) was selected as a cross-linking agent to conjugate DOX and PEI. Then, DOX–PEI (DP) conjugate was synthesized, following being condensed the anionic siRNA and charged with negatively charged BMs, resulting in the construction of BMs/DP/siRNA. Furthermore, the release of various therapeutic agents in vitro and their synergistic antitumor efficacy were systematically investigated.

Materials and methods

Materials

BMs obtained from Magnetospirillum gryphiswaldense MSR-1 were a gift from Professor Li Ying and Jiang Wei (Department of Microbiology, China Agricultural University). DOX·HCl was purchased from Dalian Meilun Biology Technology Co., Ltd. (Shanghai, China). SANH was obtained from Dernopharm Co., Ltd. (Nanjing, China). Branched PEI (molecular weight: 25 kDa) was purchased from Sigma-Aldrich Co., (St Louis, MO, USA). STAT 3 siRNA and siRNA (NC) were obtained from GenePharma Co., Ltd. (Shanghai, China), and siRNA (NC) was the negative control of STAT 3 siRNA without homology. The HeLa cervical carcinoma cell line was from Typical China Academy Culture Preservation Committee Cell Library (Shanghai, China). Cell culture medium was composed of DMEM supplemented with 10% FBS. All cells were incubated at 37°C in humidified air with 5% CO2.

Synthesis and characterization of DOX–PEI conjugates

The synthesis of DOX–PEI conjugates was carried out in two steps (Figure 1). First, SANH-PEI was conjugated by the reaction of N-N-hydroxysuccinimide (NHS) groups of SANH with the amine groups of PEI. Briefly, SANH was initially dissolved in dimethyl sulfoxide and PEI was then added to the mixture. Following that, the mixture was added to the modified buffer (0.877% sodium chloride, 1.639% sodium phosphate, pH 7.4) and stirred for 48 h under nitrogen atmosphere. The resultant was then purified by dialysis (molecular weight: 8,000–14,000). After lyophilizing, the product was confirmed by 1H nuclear magnetic resonance (NMR) (CD3SOCD3, 400 MHZ). Second, DOX–PEI was obtained by the reaction between ketone groups of DOX and hydrazine groups of SANH–PEI resulting in the hydrazone bond formation. DOX and SANH–PEI were dissolved in conjugation buffer (0.877% sodium chloride, 1.639% sodium phosphate, pH 6.0) and stirred for 48 h in the dark under nitrogen supply. The product was recovered after purification by dialysis followed by lyophilization. Then, 1H NMR (CD3SOCD3, 400 MHZ) was used to confirm the structure of DOX–PEI conjugate. The amount of DOX in DOX–PEI conjugate was determined by UV–vis spectrophotometry at 481 nm. The method for the determination of DOX content was in accordance with the validation requirements. In the determination process, we ensured that PEI and SANH had no interference with DOX. In pH 7.4 and 5.5 PBS buffer, the standard curve of DOX was Y=0.0200X+0.0033 (R2=0.9995) and Y=0.0173X+0.0027 (R2=0.9997), respectively.

Figure 1 Fabrication of BMs/DP/siRNA nanocomplexes.
Abbreviations: BMs, bacterial magnetosomes; DOX, doxorubicin; DP, DOX–PEI; PEI, polyethyleneimine; SANH, succinimidyl 6-hydrazinonicotinate acetone hydrazine.

pH-sensitive release of DOX

The pH-sensitive release of DOX from DP was determined in PBS buffer that was adjusted to pH of 5.5 and 7.4. Briefly, DP was dissolved in 1 mL of PBS and placed in the dialysis bag. Following that, the dialysis bag was placed in the tube containing 20 mL of PBS at different pH values. Then, the release process was performed by placing the bags in the incubator that maintained at 37°C and 60 rpm. The released amount of DOX in the solution was measured by using UV–vis spectrophotometry after incubation for 0.5, 1, 2, 4, 8, and 12 h. All assays were performed in triplicate.

Preparation and characterization of BMs/DP/siRNA nanocomplexes

BMs/DP/siRNA nanocomplexes were synthesized as shown in Figure 1. The nanocomplexes with different DP/siRNA weight ratios (ratio was set as 0, 0.13, 0.26, 0.52, 1.03, 1.29, 2.06, and 2.58) were prepared by adding appropriate amount of DP into siRNA-dispersed diethyl pyrocarbonate (DEPC) water, following that a fixed amount of BMs was added (BMs/siRNA weight ratio was 1:2). The mixture was vortexed for 2 min and incubated for 25 min at room temperature and collected. The formation of composites was determined by the gel electrophoresis assay. Briefly, the composites were added to 0.8% (w/v) agarose gel containing 1% (v/v) gel stain in tris acetate EDTA (TAE) buffer, the gel was run at 70 V for 20 min and imaged under UV transilluminator and a digital imaging system. The particle size and zeta potential of BMs/DP/siRNA were characterized by Zetasizer (ZEN3600; Malvern Instruments, Malvern, UK) in DEPC water.

To investigate whether the concentration of BMs affected the eventual formulation, BMs were treated with the DP/siRNA (DP/siRNA =2.06) complexes in DEPC water and incubated for 25 min (BMs/siRNA weight ratio was set as 1:5, 1:2, 1:1, 2:1, and 5:1). The products were characterized by agarose gel retardation assay and Zetasizer (ZEN3600).

Stability of BMs/DP/siRNA nanocomplexes

For heparin decomplexation assay, the prepared BMs/DP/siRNA nanocomplexes were mixed with heparin (heparin/siRNA weight ratio was 2, 10, 25, and 100) and incubated for 15 min. The resultants were subjected to agarose gel electrophoresis as described earlier.

For serum stability assay, after adding 50% FBS, naked siRNA and BMs/DP/siRNA nanocomplexes were incubated for 60, 90, 120, and 150 min. At predetermined intervals, the mixture was treated with heparin followed by incubation for a further 15 min. All samples were loaded on agarose gel for retardation analysis.

For enzyme stability assay, the procedure was the same as the serum stability assay, but the only difference was that naked siRNA and BMs/DP/siRNA were incubated with RNase A.

Cell viability assay

To assess the synergistic antitumor effect of BMs/DP/siRNA complexes, the cytotoxicity with different siRNA contents and incubation times was determined using the cell counting kit-8 (CCK-8) assay. HeLa cells were seeded on 96-well plates at a density of 2×104 cells/well and incubated for 24 h for proper cell attachment. Then, the media were replaced with siRNA, DOX, BMs-PEI/SANH, BMs-PEI/SANH-siRNA (STAT 3), BMs-PEI/SANH-siRNA (NC), and BMs/DP/siRNA (STAT 3) (the dosage of siRNA was set as 2.5, 5, 7.5, and 10 pmol, and the DP/siRNA ratio was 1.03, 1.29, 2.06, and 2.58) in 100 μL of serum-free DMEM for 6 h to protect siRNA from protein damage in serum and then followed by 200 μL of DMEM containing 10% FBS for further 48 h. Subsequently, the medium was treated with a CCK-8 working solution and incubated for additional 2 h.26 The absorption at 450 nm was performed using a microplate reader.

Cytotoxicity assay with different incubation times was evaluated as described earlier. Briefly, siRNA, DOX, BMs-PEI/SANH, BMs-PEI/SANH-siRNA (STAT 3), BMs-PEI/SANH-siRNA (NC), and BMs/DP/siRNA (STAT 3) (the dosage of siRNA was 7.5 pmol) were incubated with HeLa cells in 100 μL of serum-free DMEM for 6 h followed by 200 μL of DMEM containing 10% FBS for further 24, 48, and 72 h. Then, the cell viability was measured by repeating the abovementioned procedure.

Cell apoptosis assay

HeLa cells were seeded on 24-well plates at 1×105 cells/well and incubated for 24 h. Then, the cells were incubated with siRNA, DOX, BMs-PEI/SANH-siRNA, and BMs/DP/siRNA (the concentration of siRNA was 50 nM) in 0.5 mL of serum-free DMEM for 6 h followed by 48 h in 1 mL DMEM containing 10% FBS. Then, the harvested cells were washed three times with PBS, after being stained with acridine orange (AO)/ethidium bromide (EB) mixed dyes reagent for 2–3 min in the dark. Finally, the cell apoptosis was analyzed using a confocal laser scanning microscope (CLSM, TCS SP8; Leica Microsystems, Wetzlar, Germany).

Cellular uptake of BMs/DP/siRNA nanocomplexes

HeLa cells at a density of 4×105 cells/well were seeded in 35 mm glass-bottom dishes and incubated for 24 h. Then, the FAM-labeled BMs/DP/siRNA nanocomplexes were incubated with HeLa cells (the concentration of siRNA was 50 nM) according to the protocol of staining with mixed dye reagent (dye reagent 1 and dye reagent 2) for 2–3 min. At 0.5 and 6 h, the cells were washed thrice with cold PBS and fixed with 4% formaldehyde for 10 min and stained with DAPI for 10 min with intermittent PBS washes. Finally, the intracellular distribution of nanocomplexes was observed under CLSM.

Results and discussion

Synthesis and characterization of DOX–PEI conjugate

Recently, numerous studies have reported that the hydrazone bond as a pH-sensitive linkage, which could effectively deliver a chemotherapeutic agent such as DOX in tumor environment.27 While the commonly applied methods for fabricating hydrazone bonds involved complex reaction processes that utilize organic reagents and harsh conditions.28 To overcome these issues, here we used a bifunctional cross-linker containing an NHS section to incorporate PEI and a hydrazine section to conjugate with DOX. This synthesis process was simple and mild with excellent biocompatibility attribute. SANH has been an increasing interest to harness the conjugation of proteins, polypeptides, and DNA, while there are few studies on drug conjugation for its delivery.29,30

The freeze-dried resultant DP conjugate was confirmed by 1H NMR (Figure 2). From the spectrum of DP, the characteristic peak of DOX was weakly obvious, attributing to the high molecular weight of PEI (25 kDa) comparable to that of DOX (579.98). However, the characteristic peak at 4.0 ppm (the hydrogen of methoxy group of DOX) and the multiple peaks at 1.2 and 2.0 ppm (the characteristic peaks of DOX) in the spectrum of DP could demonstrate the successful conjugation of DOX and PEI. The amount of DOX in DP was 5.77%±0.08%, which was determined by using the UV–vis spectrophotometry analysis.

Figure 2 The 1H NMR spectrum of PEI, SANH, SANH–PEI, DOX, DOX–PEI.
Abbreviations: DOX, doxorubicin; NMR, nuclear magnetic resonance; PEI, polyethyleneimine; SANH, succinimidyl 6-hydrazinonicotinate acetone hydrazine.

pH-sensitive release of DOX

An ideal co-delivery system should be able to control the release of the incorporated therapeutics, herein a gene and a chemotherapeutic drug. Moreover, it is evident that hydrazone bond favorably cleaves under low pH conditions.7 Hence, we investigated the in vitro drug release in PBS buffers with different pH values using the dialysis method. The pH values were selected as they mimic the pH of blood plasma (pH 7.4) and lysosomal microenvironment in the tumor (pH 4–6).31

In vitro release results (Figure 3) showed that the DP polymer exhibited the long-term release behavior and pH-response release specifically at the pH value mimicking the tumor environment. At pH 5.5, approximately 40% of DOX was released from DP within 280 h, which was much higher than that of its release in pH 7.4. Based on these results, DP could keep stable in normal pH environment (blood plasma), which would decrease the leakage of drugs in vivo and improve the drug release performance in the tumor microenvironment.

Figure 3 The cumulative release of DP in PBS buffers adjusted to pH 5.5 and 7.4.
Abbreviation: DP, doxorubicin–polyethyleneimine.

Preparation and characterization of BMs/DP/siRNA nanocomplexes

Cationic DP condensed the anionic siRNA molecules and negatively charged with BMs based on electrostatic interaction, which resulted in BMs/DP/siRNA nanocomplexes. Hence, the use of PEI as a cross-linker provided a basis to co-deliver DOX and siRNA, which could be successfully delivered by escaping the lysosome via proton sponge effect.32 To investigate the encapsulation efficiency of nanocomplexes, different DP/siRNA weight ratios and BMs/siRNA ratios in BMs/DP/siRNA nanocomplexes were optimized by the agarose gel electrophoresis method as well as DLS detection (Figure 4). As shown in Figure 4A, the fraction of free siRNA was visualized at the low ratio and the band gradually decreased to no color with the increase in DP/siRNA ratio, indicating that siRNA was successfully bound to DP/BMs complex via electrostatic interactions at a DP/siRNA weight ratio of higher than 1.03.

Figure 4 The agarose gel electrophoresis results and characterization of BMs/DP/siRNA complexes.
Notes: (A) Gel electrophoresis results of BMs/DP/siRNA complexes with different DP/siRNA weight ratios, 1–8: DP/siRNA ratio – 0, 0.13, 0.26, 0.52, 1.03, 1.29, 2.06, and 2.58, (B) Zeta potential results of BMs/DP/siRNA complexes with different DP/siRNA weight ratios. (C) Diameter results (intensity) of BMs/DP/siRNA complexes with different DP/siRNA ratios, 1–4: DP/siRNA ratio – 1.03, 1.29, 2.06, and 2.58. (D) Gel images of BMs/DP/siRNA complexes with different BMs/siRNA weight ratios, 1–5: BMs/siRNA weight ratio – 1:5, 1:2, 1:1, 2:1, and 5:1. (E) Zeta potential results of BMs/DP/siRNA complexes with different BMs/siRNA weight ratios. (F) Diameter results (intensity) of BMs/DP/siRNA complexes with different BMs/siRNA ratios, 1–5: BMs/siRNA weight ratio – 1:5, 1:2, 1:1, 2:1, and 5:1.
Abbreviations: BMs, bacterial magnetosomes; DP, doxorubicin–polyethylenimine.

The size and zeta potential of BMs/DP/siRNA were critical parameters to be evaluated for exploring their cellular uptake efficiency. As shown in Figure 4B and C, the size distribution by the intensity of nanocomplex decreased from 325 to 118 nm with the increase in DP/siRNA ratios from 1.03 to 2.58, indicating that unbound siRNA was condensed to form much more compact nanocomplexes. Moreover, the zeta potential values of BMs/DP/siRNA nanocomplexes were in the range of 29 and 48 mV and with excellent dispersion stability. Multiple studies showed that nanoparticle within 200 nm could promote the accumulation due to enhanced permeability and retention effect in tumor tissue.13 In addition, a modest positive potential would significantly increase the interactions with the negatively charged cell membrane.33 Therefore, DP/siRNA ratio of 2.06 was selected as the optimal value for further studies.

To obtain the optimal content of BMs, the gel images of BMs/DP/siRNA complexes with different BMs/siRNA weight ratios were investigated (Figure 4D). It is evident from the results that the free siRNA could not be observed, indicating that the amounts of BMs had a little effect on the formation of nanocomposites. From Figure 4E and F, when the BMs/siRNA weight ratio was 1:5, the size (distribution by intensity) was relatively small (107 nm) and the potential value was positive (31.1±1.01 mV), which was significantly advantageous to augment the contact of nanocomplexes with the cells and increase their cellular internalization efficiency.

Stability of BMs/DP/siRNA nanocomplexes

As shown in Figure 5A, the siRNA from BMs/DP/siRNA composites was released when mixed with heparin, which might be due to the stronger interaction of heparin with the nanocomplexes.34 Moreover, at the heparin/siRNA weight ratio of 100 and incubation time of 15 min, the siRNA was completely dissociated from the complex and resulted in the formation of a gel.

Figure 5 Agarose gel electrophoresis assay of BMs/DP/siRNA complexes.
Notes: (A) The heparin decomplexation assay of BMs/DP/siRNA. 1: BMs/DP/siRNA and 2–5: heparin/siRNA weight ratio was 2, 10, 25, and 100. (B) The serum stability of BMs/DP/siRNA. 1: Naked siRNA incubation with 50% serum for 60 min and 2–5: BMs/DP/siRNA incubation with 50% serum for 60, 90, 120, and 150 min. (C) The enzyme stability of BMs/DP/siRNA. 1: Naked siRNA incubation with RNase A for 60 min and 2–5: BMs/DP/siRNA incubation with RNase A for 60, 90, 120, and 150 min.
Abbreviations: BMs, bacterial magnetosomes; DP, doxorubicin–polyethyleneimine.

To evaluate the stability in physiological fluids, nanocomplexes were incubated in 50% FBS (Figure 5B). Noticeably, the naked siRNA was degraded within 60 min while BMs/DP/siRNA complex could still survive for 150 min. Moreover, the enzyme stability assay in Figure 5C also showed that the BMs/DP/siRNA complex could slow down the degradation of siRNA. Above all, the new combination delivery system could protect siRNA from degradation and increase the circulation time.

Cell viability assay

In our previous study, the BMs group maintained a high level of cell viability throughout the incubation period for 7 days based on the CCK-8 assay.35 Here, the formation of BMs/DP/siRNA nanocomplexes was achieved through electrostatic interactions involving multi-step chemical synthesis. More importantly, the prepared complexes have no ketone bonds, which might be the reason for its stability. Therefore, it is required to assess the cell viability of nanocomplexes. In a dose-dependent assay, the viability of cells in the blank vector BMs-PEI/SANH group was higher than 80%, indicating that the blank vector had no significant inhibitory effect on HeLa cells (Figure 6A). And the inhibition rate of BMs/DP/siRNA (STAT 3) complex on cell proliferation was significantly higher than that of naked siRNA. Moreover, the inhibitory effect of co-delivery of siRNA and DOX was higher than that of BMs-PEI/SANH-siRNA (STAT 3), which showed that the combination system resulted in the excellent synergistic therapeutic effect. The dose of 7.5 pmol was chosen for further investigation when the DP/siRNA ratio was 2.06, BMs/siRNA ratio was 1:5, the size was relatively small, and potential was positive. Therefore, BMs/DP/siRNA complexes could play a synergistic antitumor effect of gene therapy with chemotherapy simultaneously.

Figure 6 Cell viability assay of siRNA, DOX, BMs-PEI/SANH, BMs-PEI/SANH-siRNA (STAT 3), BMs-PEI/SANH-siRNA (NC), and BMs/DP/siRNA (STAT 3) on the growth of HeLa cells (105 cells/well).
Note: The effects of (A) contents and (B) treatment times of siRNA, DOX, BMs-PEI/SANH, BMs-PEI/SANH-siRNA (STAT 3), BMs-PEI/SANH-siRNA (NC), and BMs/DP/siRNA (STAT 3) on the growth of HeLa cells (*P<0.05; **P<0.01).
Abbreviations: BMs, bacterial magnetosomes; DOX, doxorubicin; DP, DOX–PEI; PEI, polyethyleneimine; NC, negative control; SANH, succinimidyl 6-hydrazinonicotinate acetone hydrazine.

As shown in Figure 6B, cell viabilities of most samples showed an upward trend after the transient decline, which might be that the nanocomplex could not completely inhibit the viability of cells, and the residual quantity was not enough to play the antitumor effect sequentially; thus, survival cells began to proliferate after 48 h culturing. However, BMs/DP/siRNA had generally the most significant inhibitory effect on cells within 48 h. Therefore, it could be concluded that the combination strategy performed a better synergistic efficiency.

Cell apoptosis by using AO/EB dual staining assay

As shown in Figure 7, the negative control group showed almost no obvious apoptosis. However, the siRNA- and DOX-treated cells underwent early and late apoptosis, while the BMs-PEI/SANH-siRNA group resulted in late apoptotic cells, in which the sizes were not uniform and the surface was budding. To this end, BMs/DP/siRNA induced apoptosis to most of the HeLa cells and resulted in the significant chromatin condensation, indicating that the co-delivery (BMs/DP/siRNA) yielded a synergistic antitumor efficiency over monotherapy (BMs-PEI/SANH-siRNA).

Figure 7 AO/EB-stained images of HeLa cells after culturing with fresh medium (control group), siRNA, DOX, BMs-PEI/SANH-siRNA, and BMs/DP/siRNA for 48 h.
Abbreviations: AO, acridine orange; BMs, bacterial magnetosomes; DOX, doxorubicin; DP, DOX–PEI; EB, ethidium bromide; PEI, polyethyleneimine; SANH, succinimidyl 6-hydrazinonicotinate acetone hydrazine.

Cellular uptake of BMs/DP/siRNA nanocomplexes

The delivery of active siRNA to the desired site area has remained as one of the main obstacles during the development of siRNA-based delivery platforms as only a small amount of active siRNA was delivered, and the remaining was degraded. So we examined the intracellular distribution of new co-delivery nanocomplexes by using CLSM through tracking siRNA that labeled with FAM, nucleus by DAPI staining and DP conjugate, which could be traced as DOX itself is an autofluorescence molecule.

As shown in Figure 8, the location of siRNAFAM (green) and DOX–PEI (red) of the nanocomplexes was visualized in the proximity of the nucleus of HeLa cells, which was slightly different from the results of biodistribution in the most previous studies.36 The reason behind this phenomenon might be the change in dynamics and distribution of the nanocomplexes due to BMs. Moreover, it is evident that the signs of cleavage of cell nucleus were appeared, indicating that the cells began to apoptosis due to pH-responsive release of DOX by cleaving hydrazone bond,37 utilization of PEI, which could avoid the retention of lysosome and increased drug concentration in the cell.38 Such a system played an important role in combination therapy, siRNA could play gene silencing effect targeted mRNA in the cell nucleus and DOX embedded into DNA resulting in the synergistic therapeutic effect within 6 h of incubation. The co-delivery system prepared in this study provided new evidence for new RNA interference mechanism and laid the foundation for excellent synergistic therapy.

Figure 8 Cellular uptake of BMs/DP/siRNA composites by CLSM when incubating for 0.5 and 6 h.
Abbreviations: BMs, bacterial magnetosomes; CLSM, confocal laser scanning microscope; DOX, doxorubicin; DP, DOX–PEI; PEI, polyethyleneimine.

Conclusion

BMs/DP/siRNAs were successfully constructed through electrostatic interactions between cationic DP with siRNA and BMs. In vitro tests illustrated that BMs/DP conjugate with hydrazone bond provided the pH-response release behavior in a sustained fashion. Moreover, the optimal BMs/DP/siRNA could protect siRNA from degradation in serum and RNase A. Particularly, the designed BMs/DP/siRNA system achieved an excellent synergistic efficacy compared to that of siRNA alone, which might be a promising therapeutic strategy for disease treatment. However, further investigations relevant to anticancer efficacy in vivo including acute toxicity test, immune toxicity test, metabolic distribution, and tumor inhibition as well as related protein expression are required to be evaluated. Overall, the study has initially demonstrated that the pre-designed BMs/DP/siRNA nano-carrier is promising for co-delivery of a pH-sensitive chemotherapeutic agent and stable siRNA to cancer cells, and could be utilized for superior therapeutic response via combination therapy.

Acknowledgments

Financial support from National marine economic innovation and development project (16PYY007SF17), the Science Research Foundation of National Health and Family Planning Commission of PRC & United Fujian Provincial Health and Education Project for Tacking the Key Research (WKJ2016-2-22), the Program for New Century Excellent Talents in Fujian Province University (2014FJ-NCET-ZR01), and the Promotion Program for Young and Middle-aged Teachers in Science and Technology Research of Huaqiao University (ZQN-PY108) is gratefully acknowledged. This research was also supported by the Program for Innovative Research Team in Science and Technology in Fujian Province University. In addition, we must thank Ranjith Kumar Kankala and Mingzhi Zhu (University of Huaqiao University) for the revision and suggestions of our manuscript.

Disclosure

The authors report no conflicts of interest in this work.


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