Back to Journals » International Journal of Nanomedicine » Volume 6

Association between dental pulp stones and calcifying nanoparticles

Authors Zeng, Yang, Zhang, Gong, Du, Ling

Published 7 January 2011 Volume 2011:6 Pages 109—118


Review by Single-blind

Peer reviewer comments 4

Jinfeng Zeng1*, Fang Yang1*, Wei Zhang2, Qimei Gong1, Yu Du1, Junqi Ling1
1Department of Operative Dentistry and Endodontics, Guanghua School and Hospital of Stomatology and Institute of Stomatological Research, Sun Yat-sen University, Guangzhou, China; 2National Neuroscience Institute, Singapore *These authors contributed equally to this work

Abstract: The etiology of dental pulp stones, one type of extraskeletal calcification disease, remains elusive to date. Calcifying nanoparticles (CNPs), formerly referred to as nanobacteria, were reported to be one etiological factor in a number of extraskeletal calcification diseases. We hypothesized that CNPs are involved in the calcification of the dental pulp tissue, and therefore investigated the link between CNPs and dental pulp stones. Sixty-five freshly collected dental pulp stones, each from a different patient, were analyzed. Thirteen of the pulp stones were examined for the existence of CNPs in situ by immunohistochemical staining (IHS), indirect immunofluorescence staining (IIFS), and transmission electron microscope (TEM). The remaining 52 pulp stones were used for isolation and cultivation of CNPs; the cultured CNPs were identified and confirmed via their shape and growth characteristics. Among the dental pulp stones examined in situ, 84.6% of the tissue samples staines positive for CNPs antigen by IHS; the corresponding rate by IIFS was 92.3 %. In 88.2% of the cultured samples, CNPs were isolated and cultivated successfully. The CNPs were visible under TEM as 200–400 nm diameter spherical particles surrounded by a compact crust. CNPs could be detected and isolated from a high percentage of dental pulp stones, suggesting that CNPs might play an important role in the calcification of dental pulp.

Keywords: calcifying nanoparticles, nanobacteria, pulp stones

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]


Other article by this author:

Readers of this article also read:

Gemcitabine-loaded liposomes: rationale, potentialities and future perspectives

Federico C, Morittu VM, Britti D, Trapasso E, Cosco D

International Journal of Nanomedicine 2012, 7:5423-5436

Published Date: 1 November 2012

Nucleocytoplasmic transport blockage by SV40 peptide-modified gold nanoparticles induces cellular autophagy

Tsai TL, Hou CC, Wang HC, Yang ZS, Yeh CS, Shieh DB, Su WC

International Journal of Nanomedicine 2012, 7:5215-5234

Published Date: 8 October 2012

Nanofibrous poly(lactide-co-glycolide) membranes loaded with diamond nanoparticles as promising substrates for bone tissue engineering

Parizek M, Douglas TEL, Novotna K, Kromka A, Brady MA, Renzing A, Voss E, Jarosova M, Palatinus L, Tesarek P, Ryparova P, Lisa V, dos Santos AM, Bacakova L

International Journal of Nanomedicine 2012, 7:1931-1951

Published Date: 17 April 2012

Entrapment in phospholipid vesicles quenches photoactivity of quantum dots

Generalov R, Kavaliauskiene S, Westrøm S, Chen W, Kristensen S, Juzenas P

International Journal of Nanomedicine 2011, 6:1875-1888

Published Date: 7 September 2011

Evaluation of injectable silica-embedded nanohydroxyapatite bone substitute in a rat tibia defect model

Xu W, Ganz C, Weber U, Adam M, Holzhüter G, Wolter D, Frerich B, Vollmar B, Gerber T

International Journal of Nanomedicine 2011, 6:1543-1552

Published Date: 2 August 2011