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ArtinM Grafted Phospholipid Nanoparticles for Enhancing Antibiotic Cellular Uptake Against Intracellular Infection

Authors Suciati T, Nafisa S, Nareswari TL, Juniatik M, Julianti E, Wibowo MS, Yudhistira T, Ihsanawati I, Triyani Y, Khairurrijal K

Received 7 August 2020

Accepted for publication 25 September 2020

Published 10 November 2020 Volume 2020:15 Pages 8829—8843

DOI https://doi.org/10.2147/IJN.S275449

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Dr Phong A Tran


Tri Suciati,1 Safira Nafisa,2 Tantri Liris Nareswari,1 Meta Juniatik,1 Elin Julianti,1 Marlia Singgih Wibowo,1 Titah Yudhistira,3 Ihsanawati Ihsanawati,4 Yani Triyani,5 Khairurrijal Khairurrijal4,6

1School of Pharmacy, Bandung Institute of Technology, Bandung, Indonesia; 2Faculty of Pharmacy, Pancasila University, Jakarta, Indonesia; 3Faculty of Industrial Technology, Bandung Institute of Technology, Bandung, Indonesia; 4Faculty of Mathematics and Natural Sciences, Bandung Institute of Technology, Bandung, Indonesia; 5Faculty of Medicine, Bandung Islamic University, Bandung, Indonesia; 6Bioscience and Biotechnology Research Center, Bandung Institute of Technology, Bandung, Indonesia

Correspondence: Tri Suciati
School of Pharmacy, Bandung Institute of Technology, Yusuf Panigoro Building, Ganesa 10, Bandung 40132, Indonesia
Tel/Fax +62 22 2504852
Email tri.suciati@fa.itb.ac.id

Background and Aim: An antimicrobial delivery in the form of surface-modified lectin of lipid nanoparticles was proposed to improve cellular accumulation. ArtinM, an active toll-like receptor 2 (TLR2) agonist lectin isolated from cempedak (Arthocarpus integrifolia) seeds, was selected to induce cellular engulfment of nanoparticles within infected host cells.
Materials and Methods: Lipid nanoparticles were prepared using the emulsification technique before electrostatic adsorption of artinM. The formula comprising of rifampicin, soy phospholipid, and polysorbate 80 was optimized by Box-Behnken design to produce the desired particle size, entrapment efficiency, and drug loading. The optimum formula was characterized for morphology, in vitro release, and cellular transport.
Results and Discussion: Soy phospholipid showed a profound effect on controlling drug loading and entrapment efficiency. Owing to its surface activity, polysorbate 80 contributed significantly to reduce particle size; however, a higher ratio to lipid concentration resulted in a decrease of rifampicin encapsulation. The adsorption of artinM on the surface of nanoparticles was accomplished by electrostatic binding at pH 4, where this process maintained the stability of encapsulated rifampicin. A high proportion of artinM adsorbed on the surface of the nanoparticles shown by haemagglutination assay, zeta potential measurement, and transmission electron microscopy imaging. Cellular uptake revealed by confocal microscopy showed the success in transporting Nile-red labelled nanoparticles across fibroblast cells.
Conclusion: The delivery system of nanoparticles bearing artinM becomes a potential platform technology for antibiotic targeting in the treatment of life-threatening chronic diseases caused by intracellular infections.

Keywords: surface modification, Box–Behnken design, antibiotic, TLR2 agonists, botanical lectin, electrostatic binding

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