Back to Browse Journals » International Journal of Nanomedicine » Volume 2 » Issue 2

Quantum dot-doped silica nanoparticles as probes for targeting of T-lymphocytes

Authors Massimo Bottini, Federica D’Annibale, Andrea Magrini, Fabio Cerignoli, Yutaka Arimura, Marcia I Dawson, Enrico Bergamaschi, Nicola Rosato, Antonio Bergamaschi, Tomas Mustelin

Published Date August 2007 Volume 2007:2(2) Pages 227—233

DOI

Published 16 August 2007

Massimo Bottini1,2,4, Federica D’Annibale2, Andrea Magrini2, Fabio Cerignoli1, Yutaka Arimura1, Marcia I Dawson1, Enrico Bergamaschi5, Nicola Rosato3, Antonio Bergamaschi1, Tomas Mustelin1

1Burnham Institute for Medical Research, La Jolla, CA, USA; 2NAST and Department of Environmental, Occupational and Social Medicine, University of Rome Tor Vergata, Rome, Italy; 3NAST and Department of Experimental Medicine and Biochemical Sciences, University of Rome Tor Vergata, Rome, Italy; 4INFN, Laboratori Nazionali di Frascati, Frascati, Italy; 5Department of Clinical Medicine, Nephrology and Health Sciences, University of Parma, Parma, Italy

Abstract: To enhance diagnostic or therapeutic efficacy, novel nanomaterials must be engineered to function in biologically relevant environments, be visible by conventional fluorescent microscopy, and have multivalent loading capacity for easy detection or effective drug delivery. Here we report the fabrication of silica nanoparticles doped with quantum dots and superficially functionalized with amino and phosphonate groups. The amino groups were acylated with a water-soluble biotin-labeling reagent. The biotinylated nanoparticles were subsequently decorated with neutravidin by exploiting the strong affinity between neutravidin and biotin. The resultant neutravidin-decorated fluorescent silica nanoparticles stably dispersed under physiological conditions, were visible by conventional optical and confocal fluorescent microscopy, and could be further functionalized with macromolecules, nucleic acids, and polymers. We also coated the surface of the nanoparticles with biotinylated mouse anti-human CD3 (αCD3). The resultant fluorescent nanoassembly was taken up by Jurkat T cells through receptor-mediated endocytosis and was partially released to lysosomes. Thus, quantum dotdoped silica nanoparticles decorated with neutravidin represent a potentially excellent scaffold for constructing specific intracellular nanoprobes and transporters.

Keywords: silica nanoparticles, neutravidin, surface functionalization, endocytosis, intracellular nanoprobe

Download Article [PDF] 

Readers of this article also read:

Continuation of bevacizumab and addition of hormone therapy following weekly paclitaxel therapy in HER2-negative metastatic breast cancer

Redondo A, Martínez V, Zamora P, Castelo B, Pinto A, Cruz P, Higuera O, Mendiola M, Hardisson D, Espinosa E

OncoTargets and Therapy 2014, 7:2175-2181

Published Date: 27 November 2014

Chorea disclosing a polycythemia vera

Liu GD, Chang J, Liu ZJ, Qiang Q, Gu CH, Zhang YY, Wei WS

Neuropsychiatric Disease and Treatment 2014, 10:563-565

Published Date: 5 April 2014

Superparamagnetic iron oxide nanoparticles conjugated with epidermal growth factor (SPION–EGF) for targeting brain tumors

Shevtsov MA, Nikolaev BP, Yakovleva LY, Marchenko YY, Dobrodumov AV, Mikhrina AL, Martynova MG, Bystrova OA, Yakovenko IV, Ischenko AM

International Journal of Nanomedicine 2014, 9:273-287

Published Date: 3 January 2014

Quality of life measurement and outcome in aphasia

Spaccavento S, Craca A, Del Prete M, Falcone R, Colucci A, Di Palma A, Loverre A

Neuropsychiatric Disease and Treatment 2014, 10:27-37

Published Date: 17 December 2013

Targeted delivery of tissue plasminogen activator by binding to silica-coated magnetic nanoparticle

Chen JP, Yang PC, Ma YH, Tu SJ, Lu YJ

International Journal of Nanomedicine 2012, 7:5137-5149

Published Date: 27 September 2012

Once-daily treatment of ADHD with guanfacine: patient implications

Brandon C Strange

Neuropsychiatric Disease and Treatment 2008, 4:499-506

Published Date: 30 June 2008

Cellular response to low adhesion nanotopographies

Matthew J Dalby

International Journal of Nanomedicine 2007, 2:373-381

Published Date: 10 October 2007

Gadonanotubes as new high-performance MRI contrast agents

Balaji Sitharaman, Lon J Wilson

International Journal of Nanomedicine 2006, 1:291-295

Published Date: 7 May 2006

Nanomed Conference 2006 ||FREE ABSTRACTS||

Abstracts of papers presented at the Nanomed Conference, 27 and 28 June 2006, Newcastle upon Tyne, UK

International Journal of Nanomedicine 2006, 1:367-371

Published Date: 7 May 2006