Back to Browse Journals » International Journal of Nanomedicine » Volume 2 » Issue 2

Quantum dot-doped silica nanoparticles as probes for targeting of T-lymphocytes

Authors Massimo Bottini, Federica D’Annibale, Andrea Magrini, Fabio Cerignoli, Yutaka Arimura, Marcia I Dawson, Enrico Bergamaschi, Nicola Rosato, Antonio Bergamaschi, Tomas Mustelin

Published 15 July 2007 Volume 2007:2(2) Pages 227—233

Massimo Bottini1,2,4, Federica D’Annibale2, Andrea Magrini2, Fabio Cerignoli1, Yutaka Arimura1, Marcia I Dawson1, Enrico Bergamaschi5, Nicola Rosato3, Antonio Bergamaschi1, Tomas Mustelin1

1Burnham Institute for Medical Research, La Jolla, CA, USA; 2NAST and Department of Environmental, Occupational and Social Medicine, University of Rome Tor Vergata, Rome, Italy; 3NAST and Department of Experimental Medicine and Biochemical Sciences, University of Rome Tor Vergata, Rome, Italy; 4INFN, Laboratori Nazionali di Frascati, Frascati, Italy; 5Department of Clinical Medicine, Nephrology and Health Sciences, University of Parma, Parma, Italy

Abstract: To enhance diagnostic or therapeutic efficacy, novel nanomaterials must be engineered to function in biologically relevant environments, be visible by conventional fluorescent microscopy, and have multivalent loading capacity for easy detection or effective drug delivery. Here we report the fabrication of silica nanoparticles doped with quantum dots and superficially functionalized with amino and phosphonate groups. The amino groups were acylated with a water-soluble biotin-labeling reagent. The biotinylated nanoparticles were subsequently decorated with neutravidin by exploiting the strong affinity between neutravidin and biotin. The resultant neutravidin-decorated fluorescent silica nanoparticles stably dispersed under physiological conditions, were visible by conventional optical and confocal fluorescent microscopy, and could be further functionalized with macromolecules, nucleic acids, and polymers. We also coated the surface of the nanoparticles with biotinylated mouse anti-human CD3 (αCD3). The resultant fluorescent nanoassembly was taken up by Jurkat T cells through receptor-mediated endocytosis and was partially released to lysosomes. Thus, quantum dotdoped silica nanoparticles decorated with neutravidin represent a potentially excellent scaffold for constructing specific intracellular nanoprobes and transporters.

Keywords: silica nanoparticles, neutravidin, surface functionalization, endocytosis, intracellular nanoprobe

Download Article [PDF] 

Readers of this article also read:

Nanolipodendrosome-loaded glatiramer acetate and myogenic differentiation 1 as augmentation therapeutic strategy approaches in muscular dystrophy

Afzal E, Zakeri S, Keyhanvar P, Bagheri M, Mahjoubi P, Asadian M, Omoomi N, Dehqanian M, Ghalandarlaki N, Darvishmohammadi T, Farjadian F, Golvajoee MS, Afzal S, Ghaffari M, Cohan RA, Gravand A, Ardestani MS

International Journal of Nanomedicine 2013, 8:2943-2960

Published Date: 8 August 2013

Enhanced in vivo osteogenesis by nanocarrier-fused bone morphogenetic protein-4

Shiozaki Y, Kitajima T, Mazaki T, Yoshida A, Tanaka M, Umezawa A, Nakamura M, Yoshida Y, Ito Y, Ozaki T, Matsukawa A

International Journal of Nanomedicine 2013, 8:1349-1360

Published Date: 9 April 2013

Doxorubicin-mediated radiosensitivity in multicellular spheroids from a lung cancer cell line is enhanced by composite micelle encapsulation

Xu WH, Han M, Dong Q, Fu ZX, Diao YY, Liu H, Xu J, Jiang HL, Zhang SZ, Zheng S, Gao JQ, Wei QC

International Journal of Nanomedicine 2012, 7:2661-2671

Published Date: 29 May 2012

Premorbid intelligence of inpatients with different psychiatric diagnoses does not differ

Paolo Stratta, Ilaria Riccardi, Annarita Tomassini, Maria Marronaro, Roberta Pacifico, Alessandro Rossi

Neuropsychiatric Disease and Treatment 2008, 4:1241-1244

Published Date: 5 December 2008

“Getting physical”: the management of neuropsychiatric disorders using novel physical treatments

Gin S Malhi, Colleen Loo, Catherine M Cahill, Jim Lagopoulos, Philip Mitchell, Perminder Sachdev

Neuropsychiatric Disease and Treatment 2006, 2:165-179

Published Date: 15 June 2006

Plant sterols as dietary adjuvants in the reduction of cardiovascular risk: theory and evidence

Craig S Patch, Linda C Tapsell, Peter G Williams, Michelle Gordon

Vascular Health and Risk Management 2006, 2:157-162

Published Date: 15 June 2006

Updated overview of the putative role of the serotoninergic system in obsessive-compulsive disorder

Bruno Aouizerate, Dominique Guehl, Emmanuel Cuny, Alain Rougier, Pierre Burbaud, Jean Tignol, Bernard Bioulac

Neuropsychiatric Disease and Treatment 2005, 1:231-243

Published Date: 15 October 2005