Back to Browse Journals » International Journal of Nanomedicine » Volume 1 » Issue 2

Spatial control over cell attachment by partial solvent entrapment of poly lysine in microfluidic channels

Authors Nicki K Baman, Galen B Schneider, Treniece L Terry, Rebecca Zaharias, Aliasger K Salem

Published 15 June 2006 Volume 2006:1(2) Pages 213—217

Nicki K Baman1, Galen B Schneider2, Treniece L Terry3, Rebecca Zaharias2, Aliasger K Salem1,3,4


1Department of Biomedical Engineering, College of Engineering, 2Department of Prosthodontics and Dows Institute for Dental Research, College of Dentistry, 3Department of Chemical and Biochemical Engineering, College of Engineering, 4Division of Pharmaceutics, College of Pharmacy, University of Iowa, Iowa City, IA, USA



Abstract:  We demonstrate spatial control over cell attachment on biodegradable surfaces by flowing cell adhesive poly (D-lysine) (PDL) in a trifluoroethanol (TFE)–water mixture through microfluidic channels placed on a biodegradable poly (lactic acid)–poly (ethylene glycol) (PLA–PEG) substrate. The partial solvent mixture swells the PLA–PEG within the confines of the microfluidic channels allowing PDL to diffuse on to the surface gel layer. When excess water is flowed through the channels substituting the TFE–water mixture, the swollen PLA surface collapses, entrapping PDL polymer. Results using preosteoblast human palatal mesenchymal cells (HEPM) indicate that this new procedure can be used for facile attachment of cells in localized regions. The PEG component of the PLA–PEG copolymer prevents cells from binding to the nonpatterned regions.
Keywords: microfluidic, cell binding, tissue engineering, solvent entrapment

Download Article [PDF] 

Readers of this article also read:

Targeted delivery of let-7a microRNA encapsulated ephrin-A1 conjugated liposomal nanoparticles inhibit tumor growth in lung cancer

Lee HY, Mohammed KA, Kaye F, Sharma P, Moudgil BM, Clapp WL, Nasreen N

International Journal of Nanomedicine 2013, 8:4481-4494

Published Date: 21 November 2013

The role of inflammation in diabetic cardiomyopathy

Nunes S, Soares E, Pereira FC, Reis F

International Journal of Interferon, Cytokine and Mediator Research 2012, 4:59-73

Published Date: 24 July 2012

Poly(L-histidine)-tagged 5-aminolevulinic acid prodrugs: new photosensitizing precursors of protoporphyrin IX for photodynamic colon cancer therapy

Johnson RP, Chung CW, Jeong YI, Kang DH, Suh H, Kim I

International Journal of Nanomedicine 2012, 7:2497-2512

Published Date: 17 May 2012

Preparation and biodistribution of 188Re-labeled folate conjugated human serum albumin magnetic cisplatin nanoparticles (188Re-folate-CDDP/HSA MNPs) in vivo

Tang QS, Chen DZ, Xue WQ, Xiang JY, Gong YC, Zhang L, Guo CQ

International Journal of Nanomedicine 2011, 6:3077-3085

Published Date: 30 November 2011

Aberrant positioning of a central venous dialysis catheter to reveal a left-sided partial anomalous pulmonary venous connection

Manohar R Chintu, Shammikumar Chinnappa, Sunil Bhandari

Vascular Health and Risk Management 2008, 4:1141-1143

Published Date: 10 October 2008

Editorial: Developing better therapies through collegial interaction ||Free Paper||

Richard F Walker

Clinical Interventions in Aging 2007, 2:273-274

Published Date: 15 October 2007

Recognition of transorbital intracranial injury ||FREE PAPER||

Roger E Turbin, Flora Levin, Dawn N Maxwell

Clinical Ophthalmology 2007, 1:85-89

Published Date: 15 June 2007

Silicon nanoparticles: applications in cell biology and medicine

Norah O’Farrell, Andrew Houlton, Benjamin R Horrocks

International Journal of Nanomedicine 2006, 1:451-472

Published Date: 15 January 2007