Back to Browse Journals » International Journal of Nanomedicine » Volume 1 » Issue 2

Spatial control over cell attachment by partial solvent entrapment of poly lysine in microfluidic channels

Authors Nicki K Baman, Galen B Schneider, Treniece L Terry, Rebecca Zaharias, Aliasger K Salem

Published Date January 2006 Volume 2006:1(2) Pages 213—217

DOI

Published 25 January 2006

Nicki K Baman1, Galen B Schneider2, Treniece L Terry3, Rebecca Zaharias2, Aliasger K Salem1,3,4

 

1Department of Biomedical Engineering, College of Engineering, 2Department of Prosthodontics and Dows Institute for Dental Research, College of Dentistry, 3Department of Chemical and Biochemical Engineering, College of Engineering, 4Division of Pharmaceutics, College of Pharmacy, University of Iowa, Iowa City, IA, USA

 

 

Abstract:  We demonstrate spatial control over cell attachment on biodegradable surfaces by flowing cell adhesive poly (D-lysine) (PDL) in a trifluoroethanol (TFE)–water mixture through microfluidic channels placed on a biodegradable poly (lactic acid)–poly (ethylene glycol) (PLA–PEG) substrate. The partial solvent mixture swells the PLA–PEG within the confines of the microfluidic channels allowing PDL to diffuse on to the surface gel layer. When excess water is flowed through the channels substituting the TFE–water mixture, the swollen PLA surface collapses, entrapping PDL polymer. Results using preosteoblast human palatal mesenchymal cells (HEPM) indicate that this new procedure can be used for facile attachment of cells in localized regions. The PEG component of the PLA–PEG copolymer prevents cells from binding to the nonpatterned regions.
Keywords: microfluidic, cell binding, tissue engineering, solvent entrapment

Download Article [PDF] 

Readers of this article also read:

Level of agreement between self-rated and clinician-rated instruments when measuring major depressive disorder in the Thai elderly: a 1-year assessment as part of the THAISAD study

Wongpakaran N, Wongpakaran T, Wannarit K, Saisavoey N, Pinyopornpanish M, Lueboonthavatchai P, Apisiridej N, Srichan T, Ruktrakul R, Satthapisit S, Nakawiro D, Hiranyatheb T, Temboonkiat A, Tubtimtong N, Rakkhajeekul S, Wongtanoi B, Tanchakvaranont S, Bookkamana P, Srisutasanavong U, Nivataphand R, Petchsuwan D

Clinical Interventions in Aging 2014, 9:377-382

Published Date: 25 February 2014

Evaluation of glycophenotype in breast cancer by quantum dot-lectin histochemistry

Andrade CG, Cabral Filho PE, Tenorio DPL, Santos BS, Beltrão EIC, Fontes A, Carvalho Jr LB

International Journal of Nanomedicine 2013, 8:4623-4629

Published Date: 2 December 2013

Polyacrylonitrile nanofibers coated with silver nanoparticles using a modified coaxial electrospinning process

Yu DG, Zhou J, Chatterton NP, Li Y, Huang J, Wang X

International Journal of Nanomedicine 2012, 7:5725-5732

Published Date: 12 November 2012

Synthesis of silver nanoparticles by using tea leaf extract from Camellia Sinensis

Loo YY, Chieng BW, Nishibuchi M, Radu S

International Journal of Nanomedicine 2012, 7:4263-4267

Published Date: 2 August 2012

Preventing postoperative abdominal adhesions in a rat model with PEG-PCL-PEG hydrogel

Yang B, Gong C, Zhao X, Zhou S, Li Z, Qi X, Zhong Q, Luo F, Qian Z

International Journal of Nanomedicine 2012, 7:547-557

Published Date: 2 February 2012

Frequency and clinical relevance of human bocavirus infection in acute exacerbations of chronic obstructive pulmonary disease

Felix C Ringshausen, Ai-Yui M Tan, Tobias Allander, Irmgard Borg, Umut Arinir, et al

International Journal of Chronic Obstructive Pulmonary Disease 2009, 4:111-117

Published Date: 4 February 2009