Back to Browse Journals » International Journal of Nanomedicine » Volume 5

Nanoparticulate Quillaja saponin induces apoptosis in human leukemia cell lines with a high therapeutic index

Authors Kefei Hu, Saideh Berenjian, Rolf Larsson, et al

Published 6 January 2010 Volume 2010:5 Pages 51—62

DOI http://dx.doi.org/10.2147/IJN.S7879

Review by Single-blind

Peer reviewer comments 5

Kefei Hu1, Saideh Berenjian1, Rolf Larsson2, Joachim Gullbo2, Peter Nygren3, Tanja Lövgren4, Bror Morein1

1Department of Medical Sciences, Section of Virology, Uppsala University, Uppsala, Sweden; 2Department of Medical Sciences, Uppsala University Hospital, Uppsala, Sweden; 3Department of Oncology, Radiology and Clinical Immunology, Uppsala University Hospital, Uppsala, Sweden; 4Department of Molecular Bioscience, Section for Veterinary Immunology and Virology, Swedish University of Agricultural Sciences, Uppsala, Sweden

Abstract: Saponin fractions of Quillaja saponaria Molina (QS) have cytotoxic activity against cancer cells in vitro, but are too toxic to be useful in the clinic. The toxic effect was abolished by converting QS fractions into stable nanoparticles through the binding of QS to cholesterol. Two fractions of QS were selected for particle formation, one with an acyl-chain (ASAP) was used to form killing and growth-inhibiting (KGI) particles, and the other without the acyl-chain (DSAP) was used to formulate blocking and balancing effect (BBE) particles. KGI showed significant growth inhibiting and cancer cell-killing activities in nine of 10 cell lines while BBE showed that on one cell line. The monoblastoid lymphoma cell line U937 was selected for analyzing the mode of action. Low concentrations of KGI (0.5 and 2 µg/mL) induced irreversible exit from the cell cycle, differentiation measured by cytokine production, and eventually programmed cell death (apoptosis). Compared to normal human monocytes, the U937 cells were 30-fold more sensitive to KGI. The nontoxic BBE blocked the cell killing effect of KGI in a concentrationdependent manner. In conclusion, the formulation of QS into nanoparticles has the potential of becoming a new class of anticancer agents.

Keywords: anticancer drug, Quillaja saponin, nanoparticle, apoptosis

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF] 

 

Readers of this article also read:

Predictors of quality of life among individuals with schizophrenia

Suttajit S, Pilakanta S

Neuropsychiatric Disease and Treatment 2015, 11:1371-1379

Published Date: 28 May 2015

Epidemiology of uveitis in the mid-Atlantic United States

Bajwa A, Osmanzada D, Osmanzada S, Khan I, Patrie J, Xin W, Reddy AK

Clinical Ophthalmology 2015, 9:889-901

Published Date: 20 May 2015

Awareness and predictors of female genital mutilation/cutting among young health advocates

Abolfotouh SM, Ebrahim AZ, Abolfotouh MA

International Journal of Women's Health 2015, 7:259-269

Published Date: 20 February 2015

Vaginal Lactobacillus: biofilm formation in vivo – clinical implications

Ventolini G

International Journal of Women's Health 2015, 7:243-247

Published Date: 16 February 2015

Posttraumatic stress symptoms, dissociation, and alexithymia in an Italian sample of flood victims

Craparo G, Gori A, Mazzola E, Petruccelli I, Pellerone M, Rotondo G

Neuropsychiatric Disease and Treatment 2014, 10:2281-2284

Published Date: 28 November 2014

Organic functionalization of single-walled carbon nanotubes (SWCNTs) with some chemotherapeutic agents as a potential method for drug delivery

Ardeshir Khazaei, Mohammad Navid Soltani Rad, Maryam Kiani Borazjani

International Journal of Nanomedicine 2010, 5:639-645

Published Date: 30 August 2010

Bactericidal effect of iron oxide nanoparticles on Staphylococcus aureus

Nhiem Tran, Aparna Mir, Dhriti Mallik, et al

International Journal of Nanomedicine 2010, 5:277-283

Published Date: 14 April 2010

Biomimetic nanoparticles: preparation, characterization and biomedical applications

Ana Maria Carmona-Ribeiro

International Journal of Nanomedicine 2010, 5:249-259

Published Date: 1 April 2010

Overview of the main methods used to combine proteins with nanosystems: absorption, bioconjugation, and encapsulation

Mariagrazia Di Marco, Shaharum Shamsuddin, Khairunisak Abdul Razak, et al

International Journal of Nanomedicine 2010, 5:37-49

Published Date: 22 December 2009