Back to Browse Journals » Vascular Health and Risk Management » Volume 4 » Issue 4

Characterizing blood microparticles: Technical aspects and challenges

Authors Arun S Shet

Published 8 August 2008 Volume 2008:4(4) Pages 769—774


Arun S Shet

Department of Medicine, St. Johns Medical College and Hospital, St. Johns National Academy of Health Sciences, Bangalore, India

Abstract: Although long considered to be cellular debris, microparticles (MPs) are more recently considered refl ective of cellular stimulation, activation, and degeneration/apoptosis. MPs that arise from the cellular components of blood and the endothelial lining of blood vessels are referred to as blood MPs and by general consensus are small (≤1.5 μm), expose the anionic phospholipid (PL) phosphatidylserine (PS) on the outer leaflet of their membrane, and bear surface membrane antigens refl ecting their cellular origin. This brief review summarizes the different approaches used by several groups to study blood MPs. The aim of this article is to review the technical aspects of characterizing the morphological and functional properties of blood MPs with emphasis on the preanalytical and analytical variables involved in these studies.

Keywords: microparticles, flow cytometry, techniques

Download Article [PDF] 

Creative Commons License This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution - Non Commercial (unported, v3.0) License. The full terms of the License are available at Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. Permissions beyond the scope of the License are administered by Dove Medical Press Limited. Information on how to request permission may be found at:

Readers of this article also read:

Relationship between cobalamin deficiency and delirium in elderly patients undergoing cardiac surgery

Sevuk U, Baysal E, Ay N, Altas Y, Altindag R, Yaylak B, Alp V, Demirtas E

Neuropsychiatric Disease and Treatment 2015, 11:2033-2039

Published Date: 7 August 2015

The antitumor activity of a doxorubicin loaded, iRGD-modified sterically-stabilized liposome on B16-F10 melanoma cells: in vitro and in vivo evaluation

Yu KF, Zhang WQ, Luo LM, Song P, Li D, Du R, Ren W, Huang D, Lu WL, Zhang X, Zhang Q

International Journal of Nanomedicine 2013, 8:2473-2485

Published Date: 15 July 2013

Spectral domain optical coherence tomographic findings at convalescent stage of acute zonal occult outer retinopathy

Kouichi Ohta, Atsuko Sato Emi Fukui

Clinical Ophthalmology 2009, 3:423-428

Published Date: 20 July 2009

Misregulation of rhodopsin phosphorylation and dephosphorylation found in P23H rat retinal degeneration

Yoshiyuki Saito, Hiroshi Ohguro, Ikuyo Ohguro, Noriyuki Sato, Futoshi Ishikawa, et al

Clinical Ophthalmology 2008, 2:821-828

Published Date: 5 December 2008

Current management of type B aortic dissection

T Paul Tran, Ali Khoynezhad

Vascular Health and Risk Management 2009, 5:53-63

Published Date: 10 October 2008

Screening for popliteal aneurysms should not be a routine part of a community-based aneurysm screening program

Martin Claridge, Simon Hobbs, Clive Quick, Donald Adam, Andrew Bradbury, Teun Wilmink

Vascular Health and Risk Management 2006, 2:189-191

Published Date: 15 June 2006