Apoptosis and G2/M arrest induced by Allium ursinum (ramson) watery extract in an AGS gastric cancer cell line
Xiao-yan Xu,1 Guo-qing Song,2 Yan-qiu Yu,1 Hai-ying Ma,1 Ling Ma,1 Yu-nan Jin1
1Department of Pathophysiology, Institute of Pathology and Pathophysiology, College of Basic Medicine, China Medical University, 2Department of Gastrointestinal Surgery, Shengjing Hospital of China Medical University, Shenyang, Liaoning, People's Republic of China
Background: The present study was designed to determine whether Allium ursinum L (ramson) could inhibit the proliferation of human AGS gastric cancer cells. Furthermore, we attempted to determine whether this inhibition could occur by targeting regulatory elements of the cell cycle.
Methods: Flow cytometry was used to observe apoptosis and the cell cycle in AGS cell lines treated or not treated with ramson watery extract. Proteins related to the cell cycle were detected by Western blotting. Caspase activity was measured using a colorimetric assay kit according to the manufacturer's instructions.
Results: Ramson watery extract induced apoptosis and G2/M phase arrest in AGS cells. Western blotting showed that cyclin B was inhibited by ramson watery extract. However, G1 phase-related proteins remain unchanged after treatment.
Conclusion: Our results indicate that ramson effectively suppressed proliferation and induced apoptosis and G2/M arrest in AGS cells by regulating elements of the cell cycle.
Keywords: ramson, G2/M phase arrest, apoptosis, Allium, gastric cancer
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