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Antioxidative fullerol promotes osteogenesis of human adipose-derived stem cells

Authors Yang XL, Li CJ, Wan YP, Smith P, Shang GW, Cui Q

Received 25 April 2014

Accepted for publication 25 May 2014

Published 20 August 2014 Volume 2014:9(1) Pages 4023—4031

DOI https://doi.org/10.2147/IJN.S66785

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 3

Xinlin Yang, Ching-Ju Li, Yueping Wan, Pinar Smith, Guowei Shang, Quanjun Cui

Department of Orthopaedic Surgery, University of Virginia School of Medicine, Charlottesville, VA, USA

Abstract: Antioxidants were implicated as potential reagents to enhance osteogenesis, and nano-fullerenes have been demonstrated to have a great antioxidative capacity by both in vitro and in vivo experiments. In this study, we assessed the impact of a polyhydroxylated fullerene, fullerol, on the osteogenic differentiation of human adipose-derived stem cells (ADSCs). Fullerol was not toxic against human ADSCs at concentrations up to 10 µM. At a concentration of 1 µM, fullerol reduced cellular reactive oxygen species after a 5-day incubation either in the presence or in the absence of osteogenic media. Pretreatment of fullerol for 7 days increased the osteogenic potential of human ADSCs. Furthermore, when incubated together with osteogenic medium, fullerol promoted osteogenic differentiation in a dose-dependent manner. Finally, fullerol proved to promote expression of FoxO1, a major functional isoform of forkhead box O transcription factors that defend against reactive oxygen species in bone. Although further clarification of related mechanisms is required, the findings may help further development of a novel approach for bone repair, using combined treatment of nano-fullerol with ADSCs.

Keywords: polyhydroxylated fullerene, bone repair, reactive oxygen species, forkhead box protein O1

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