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Antimicrobial action of chlorhexidine digluconate in self-ligating and conventional metal brackets infected with Streptococcus mutans biofilm

Authors Dias AP, Paschoal MAB, Diniz RS, Lage LM, Gonçalves LM

Received 9 August 2017

Accepted for publication 18 November 2017

Published 19 April 2018 Volume 2018:10 Pages 69—74

DOI https://doi.org/10.2147/CCIDE.S148700

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Professor Christopher E. Okunseri


Ana Paula Dias, Marco Aurélio Benini Paschoal, Rafael Soares Diniz, Lucas Meneses Lage, Letícia Machado Gonçalves

Department of Dentistry, CEUMA University, São Luis, Maranhão, Brazil

Objectives: The objectives of this study were to assess the adherence of Streptococcus mutans biofilms grown over conventional ligature (CL) or self-ligating (SL) metal brackets and their bacterial viability after 0.12% chlorhexidine (CHX) digluconate treatment.
Materials and methods: The sample consisted of 48 metallic orthodontic brackets divided randomly into two groups: CL (n=24) and SL brackets (n=24). S. mutans biofilms were grown over the bracket surface (96 h) and treated with CHX (positive control) or 0.9% phosphate-buffered saline (PBS) (negative control) for 1 min each. Quantitative analysis was assessed by colony-forming units, and fluorescence microscopy was performed aiming to illustrate the outcomes. The tests were done in triplicate at three different times (n=9). Data were analyzed using ANOVA and Tukey test (P<0.05).
Results: There were significant differences in brackets’ biofilm formation, being CL largely colonized compared with SL, which was observed by colony-forming unit counting (P<0.05) and microcopy images. Significant reduction in the viability of S. mutans was found in both brackets treated with CHX compared to PBS (P<0.05).
Conclusion: The antimicrobial activities of CHX were similar for CL and SL brackets (P>0.05). In conclusion, a lower colonization was achieved in SL brackets and S. mutans biofilms were susceptible to CHX treatment to both studied brackets.

Keywords: biofilm, chlorhexidine, orthodontic brackets, Streptococcus mutans

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