Back to Journals » Journal of Experimental Pharmacology » Volume 9

Antidiabetic and gastric emptying inhibitory effect of herbal Melia azedarach leaf extract in rodent models of diabetes type 2 mellitus

Authors Seifu D, Gustafsson LE, Chawla R, Genet S, Debella A, Holst M, Hellström PM

Received 30 October 2016

Accepted for publication 27 January 2017

Published 20 March 2017 Volume 2017:9 Pages 23—29

DOI https://doi.org/10.2147/JEP.S126146

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Akshita Wason

Peer reviewer comments 2

Editor who approved publication: Professor Bal Lokeshwar

Daniel Seifu,1,2 Lars E Gustafsson,3 Rajinder Chawla,1 Solomon Genet,1 Asfaw Debella,4 Mikael Holst,5 Per M Hellström2

1Department of Biochemistry, College of Health Sciences, School of Medicine, Addis Ababa University, Addis Ababa, Ethiopia; 2Department of Medical Sciences, Uppsala University, Uppsala, 3Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden; 4Department of Traditional Drug Development, Ethiopian Health and Nutrition Research Institute, Addis Ababa, Ethiopia; 5Department of Women’s and Children’s Health, Karolinska Institutet, Stockholm, Sweden

Abstract: Diabetes type 2 is associated with impaired insulin production and increased insulin resistance. Treatment with antidiabetic drugs and insulin strives for normalizing glucose homeostasis. In Ethiopian traditional medicine, plant extracts of Melia azedarach are used to control diabetes mellitus and various gastrointestinal disorders. The objective of this study was to clarify the antidiabetic effects of M. azedarach leaf extracts in diabetic type 2 experimental animals. In this study, mice were injected with Melia extract intraperitoneally. Plasma glucose was studied by using tail vein sampling in acute experiments over 4 h and chronic experiments over 21 days with concurrent insulin and body weight assessments. Glucose tolerance was studied by using intraperitoneal glucose (2 mg/g) tolerance test over 120 min. Gastric emptying of a metabolically inert meal was studied by the gastric retention of a radioactive marker over 20 min. Melia extracts displayed acute, dose-dependent antidiabetic effects in ob/ob mice similar to glibenclamide (p<0.05–0.001). Long-term administration of Melia extract reduced plasma glucose (p<0.001) and insulin (p<0.01–0.001) levels over 21 days, concurrent with body weight loss. Glucose tolerance test showed reduced basal glucose levels (p<0.05–0.01), but no difference was found in glucose disposal after long-term treatment with Melia extract. In addition, the Melia extract at 400 mg/kg slowed gastric emptying rate of normal Sprague-Dawley (p<0.001) and diabetic Goto-Kakizaki rats (p<0.001) compared with controls. It is concluded that the M. azedarach leaf extract elicits diabetic activity through a multitargeted action. Primarily an increased insulin-sensitizing effect is at hand, resulting in blood glucose reduction and improved peripheral glucose disposal, but also through reduced gastric emptying and decreased insulin demand.

Keywords: ob/ob mouse, Sprague-Dawley, Goto-Kakizaki, glucose tolerance test

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]