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Anticancer activity of paclitaxel-loaded keratin nanoparticles in two-dimensional and perfused three-dimensional breast cancer models

Authors Foglietta F, Spagnoli GC, Muraro MG, Ballestri M, Guerrini A, Ferroni C, Aluigi A, Sotgiu G, Varchi G

Received 15 December 2017

Accepted for publication 21 April 2018

Published 29 August 2018 Volume 2018:13 Pages 4847—4867


Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 4

Editor who approved publication: Dr Thomas Webster

Federica Foglietta,1 Giulio C Spagnoli,2,3 Manuele Giuseppe Muraro,2 Marco Ballestri,4 Andrea Guerrini,4 Claudia Ferroni,4 Annalisa Aluigi,4 Giovanna Sotgiu,4 Greta Varchi4

1Department of Drug Science & Technology, University of Torino, Torino, Italy; 2Department of Biomedicine, University of Basel and University Hospital of Basel, Basel, Switzerland; 3Institute of Translational Pharmacology, CNR, Rome, Italy; 4Institute for Organic Synthesis & Photoreactivity, National Research Council, Bologna, Italy

Purpose: Taxanes are highly effective cytotoxic drugs for progressing breast cancer treatment. However, their poor solubility and high toxicity urge the development of innovative formulations of potential clinical relevance.
Materials and methods: By using a simple and straightforward aggregation method, we have generated paclitaxel (PTX) loaded in keratin nanoparticles (KER-NPs-PTX). Their activities were tested against human breast cancer MCF-7 and MDA MB 231 cell lines in conventional two-dimensional (2D) cultures and in a dynamic three-dimensional (3D) model with perfused bioreactor (p3D). Moreover, KER-NPs-PTX activity was compared to free PTX and to PTX loaded in albumin nanoparticles (HSA-NPs-PTX). Cell viability, induction of apoptosis, and gene expression analysis were used as readouts.
Results: In 2D cultures, KER-NPs-PTX was able to inhibit tumor cell viability and to induce apoptosis similarly to PTX and HSA-NPs-PTX. In the p3D model, a lower sensitivity of tumor cells to treatments was observed. Importantly, only KER-NPs-PTX was able to induce a statistically significant increase in apoptotic cell percentages following 24 h treatment for MCF-7 (16.7±4.0 early and 11.3±4.9 late apoptotic cells) and 48 h treatment for MDA MB 231 (21.3±11.2 early and 10.5±1.8 late apoptotic cells) cells. These effects were supported, at least for MCF-7 cells, by significant increases in the expression of proapoptotic BAX gene (5.8±0.5) 24 h after treatment and of cleaved caspase 3 (CC3) protein.
Conclusion: KER-NPs-PTX, generated by a simple procedure, is characterized by high water solubility and enhanced PTX-loading ability, as compared to HSA-NPs-PTX. Most importantly, it appears to be able to exert effective anticancer activities on breast cancer cells cultured in 2D or in p3D models.

Keywords: keratin, nanoparticles, paclitaxel, breast cancer, perfused three-dimensional models

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