Anti-Inflammatory Effects of Magnetically Targeted Mesenchymal Stem Cells on Laser-Induced Skin Injuries in Rats
Received 13 April 2020
Accepted for publication 13 July 2020
Published 6 August 2020 Volume 2020:15 Pages 5645—5659
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 2
Editor who approved publication: Dr Mian Wang
Xiuying Li, 1,* Zhenhong Wei, 1,* Wei Zhang, 2 Huiying Lv, 1 Jing Li, 1 Liya Wu, 1 Hao Zhang, 3 Bai Yang, 3 Mingji Zhu, 4 Jinlan Jiang 1
1Scientific Research Center, China-Japan Union Hospital of Jilin University, Changchun, Jilin, People’s Republic of China; 2Department of Radiotherapy, The Second Hospital of Jilin University, Changchun, Jilin, People’s Republic of China; 3State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun, Jilin, People’s Republic of China; 4Dermatological Department, China-Japan Union Hospital of Jilin University, Changchun, Jilin, People’s Republic of China
*These authors contributed equally to this work
Correspondence: Jinlan Jiang
Scientific Research Center, China-Japan Union Hospital of Jilin University, No. 126 Xiantai Street, Changchun 130033, Jilin, People’s Republic of China
Mingji Zhu Dermatological Department No. 126 Xiantai Street, Changchun 130033, Jilin, People’s Republic of China
Tel/Fax +86 04 318 499 5423
Introduction: Mesenchymal stem cells (MSCs) are a promising resource for tissue regeneration and repair. However, their clinical application is hindered by technical limitations related to MSC enrichment at the target sites.
Methods: MSCs were labeled with magnetic Fe 3O 4 nanoparticles (NPs). We analyzed the effects of NP on cell proliferation, stem cell characteristics, and cytokine secretion. Furthermore, we induced NP-labeled MSC migration with an external magnetic field toward laser-induced skin wounds in rats and evaluated the associated anti-inflammatory effects.
Results: Fe 3O 4 NP application did not adversely affect MSC characteristics. Moreover, Fe 3O 4 NP-labeled MSCs presented increased anti-inflammatory cytokine and chemokine production compared with unlabeled MSCs. Furthermore, MSCs accumulated at the injury site and magnetic targeting promoted NP-labeled MSC migration toward burn injury sites in vivo. On day 7 following MSC injection, reduced inflammation and promoted angiogenesis were observed in the magnetically targeted MSC group. In addition, anti-inflammatory factors were upregulated, whereas pro-inflammatory factors were downregulated within the magnetically targeted MSC group compared with those in the PBS group.
Conclusion: This study demonstrates that magnetically targeted MSCs contribute to cell migration to the site of skin injury, improve anti-inflammatory effects and enhance angiogenesis compared with MSC injection alone. Therefore, magnetically targeted MSC therapy may be an effective treatment approach for epithelial tissue injuries.
Keywords: mesenchymal stem cells, skin injury, magnetic targeting, Fe 3O 4 nanoparticle, anti-inflammatory effect
Corrigendum for this paper has been published
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