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Anti-Inflammatory Effects of Magnetically Targeted Mesenchymal Stem Cells on Laser-Induced Skin Injuries in Rats

Authors Li X, Wei Z, Zhang W, Lv H, Li J, Wu L, Zhang H, Yang B, Zhu M, Jiang J

Received 13 April 2020

Accepted for publication 13 July 2020

Published 6 August 2020 Volume 2020:15 Pages 5645—5659

DOI https://doi.org/10.2147/IJN.S258017

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Dr Mian Wang


Xiuying Li, 1,* Zhenhong Wei, 1,* Wei Zhang, 2 Huiying Lv, 1 Jing Li, 1 Liya Wu, 1 Hao Zhang, 3 Bai Yang, 3 Mingji Zhu, 4 Jinlan Jiang 1

1Scientific Research Center, China-Japan Union Hospital of Jilin University, Changchun, Jilin, People’s Republic of China; 2Department of Radiotherapy, The Second Hospital of Jilin University, Changchun, Jilin, People’s Republic of China; 3State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun, Jilin, People’s Republic of China; 4Dermatological Department, China-Japan Union Hospital of Jilin University, Changchun, Jilin, People’s Republic of China

*These authors contributed equally to this work

Correspondence: Jinlan Jiang
Scientific Research Center, China-Japan Union Hospital of Jilin University, No. 126 Xiantai Street, Changchun 130033, Jilin, People’s Republic of China
Email jiangjinlan@jlu.edu.cn
Mingji Zhu Dermatological Department No. 126 Xiantai Street, Changchun 130033, Jilin, People’s Republic of China
Tel/Fax +86 04 318 499 5423
Email zmj@jlu.edu.cn

Introduction: Mesenchymal stem cells (MSCs) are a promising resource for tissue regeneration and repair. However, their clinical application is hindered by technical limitations related to MSC enrichment at the target sites.
Methods: MSCs were labeled with magnetic Fe 3O 4 nanoparticles (NPs). We analyzed the effects of NP on cell proliferation, stem cell characteristics, and cytokine secretion. Furthermore, we induced NP-labeled MSC migration with an external magnetic field toward laser-induced skin wounds in rats and evaluated the associated anti-inflammatory effects.
Results: Fe 3O 4 NP application did not adversely affect MSC characteristics. Moreover, Fe 3O 4 NP-labeled MSCs presented increased anti-inflammatory cytokine and chemokine production compared with unlabeled MSCs. Furthermore, MSCs accumulated at the injury site and magnetic targeting promoted NP-labeled MSC migration toward burn injury sites in vivo. On day 7 following MSC injection, reduced inflammation and promoted angiogenesis were observed in the magnetically targeted MSC group. In addition, anti-inflammatory factors were upregulated, whereas pro-inflammatory factors were downregulated within the magnetically targeted MSC group compared with those in the PBS group.
Conclusion: This study demonstrates that magnetically targeted MSCs contribute to cell migration to the site of skin injury, improve anti-inflammatory effects and enhance angiogenesis compared with MSC injection alone. Therefore, magnetically targeted MSC therapy may be an effective treatment approach for epithelial tissue injuries.

Keywords: mesenchymal stem cells, skin injury, magnetic targeting, Fe 3O 4 nanoparticle, anti-inflammatory effect

Corrigendum for this paper has been published

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