Back to Journals » International Journal of Nanomedicine » Volume 9 » Issue 1

Analyzing the behavior of a porous nano-hydroxyapatite/polyamide 66 (n-HA/PA66) composite for healing of bone defects

Authors Xiong Y, Ren C, Zhang B, Yang H, Lang Y, Min L, Zhang W, Pei F, Yan Y, Li H, Mo A, Tu C, Duan H

Received 15 August 2013

Accepted for publication 11 September 2013

Published 13 January 2014 Volume 2014:9(1) Pages 485—494

DOI https://doi.org/10.2147/IJN.S52990

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 3

Yan Xiong,1 Cheng Ren,1 Bin Zhang,1 Hongsheng Yang,1 Yun Lang,1 Li Min,1 Wenli Zhang,1 Fuxing Pei,1 Yonggang Yan,2 Hong Li,2 Anchun Mo,3 Chongqi Tu,1 Hong Duan1

1Department of Orthopedics, West China Hospital, 2School of Physical Science and Technology, 3Department of Oral Implant, West China College of Stomatology, Sichuan University, Chengdu, People's Republic of China

Abstract: The aim of this study was to analyze the behavior of the porous nano-hydroxyapatite/polyamide 66 (n-HA/PA66) composite grafted for bone defect repair through a series of biological safety experiments, animal experiments, and a more than 5-year long clinical follow-up. The biological safety experiments, carried out in accordance with the Chinese Guo Biao and Tolerancing (GB/T)16886 and GB/T16175, revealed that porous n-HA/PA66 composite had no cytotoxicity, no sensitization effect, no pyrogenic reaction, and that its hemolysis rate was 0.59% (less than 5%). Rabbit models of tibia defects with grafted porous n-HA/PA66 composite were established. After 2 weeks, the experiment showed that osteogenesis was detected in the porous n-HA/PA66 composite; the density of new bone formation was similar to the surrounding host bone at 12 weeks. After 26 weeks, the artificial bone rebuilt to lamellar bone completely. In the clinical study, a retrospective review was carried out for 21 patients who underwent serial radiographic assessment after treatment with porous n-HA/PA66 composite grafts following bone tumor resection. All wounds healed to grade A. No postoperative infections, delayed deep infection, nonspecific inflammation, rejection, or fractures were encountered. At a mean follow-up of 5.3 years, the mean Musculoskeletal Tumor Society's (MSTS) 93 score was 29.3 points (range: 28–30 points) and mean radiopaque density ratio was 0.77±0.10. The radiologic analysis showed that porous n-HA/PA66 composite had been completely incorporated with the host bone about 1.5 years later. In conclusion, this study indicated that the porous n-HA/PA66 composite had biological safety, and good biocompatibility, osteoinduction, and osseointegration. Thus, the porous n-HA/PA66 composite is an ideal artificial bone substitute and worthy of promotion in the field.

Keywords: biomaterial, artificial bone materials, porous n-HA/PA66 composite (n-HA/PA66), bone defects healing, bone grafting

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]

 

Other articles by this author:

In vitro stress effect on degradation and drug release behaviors of basic fibroblast growth factor – poly(lactic-co-glycolic-acid) microsphere

Xiong Y, Yu ZP, Lang Y, Hu JY, Li H, Yan YG, Tu CQ, Yang TF, Song YM, Duan H, Pei FX

Drug Design, Development and Therapy 2016, 10:431-440

Published Date: 25 January 2016

Pelvis metastasis from primary choroidal melanoma: a case report

Xiong Y, Lang Y, Tu CQ, Duan H

OncoTargets and Therapy 2014, 7:2107-2110

Published Date: 14 November 2014

Readers of this article also read:

Skull base metastasis from papillary thyroid carcinoma: a report of three cases

Tunio MA, Al Asiri M, Al-Qahtani KH, Aldandan S, Riaz K, Bayoumi Y

International Medical Case Reports Journal 2015, 8:127-131

Published Date: 25 June 2015

Biological responses according to the shape and size of carbon nanotubes in BEAS-2B and MESO-1 cells

Haniu H, Saito N, Matsuda Y, Tsukahara T, Usui Y, Maruyama K, Takanashi S, Aoki K, Kobayashi S, Nomura H, Tanaka M, Okamoto M, Kato H

International Journal of Nanomedicine 2014, 9:1979-1990

Published Date: 17 April 2014

Inhibited bacterial biofilm formation and improved osteogenic activity on gentamicin-loaded titania nanotubes with various diameters

Lin WT, Tan HL, Duan ZL, Yue B, Ma R, He G, Tang TT

International Journal of Nanomedicine 2014, 9:1215-1230

Published Date: 7 March 2014

Ammonium glycyrrhizinate-loaded niosomes as a potential nanotherapeutic system for anti-inflammatory activity in murine models

Marianecci C, Rinaldi F, Di Marzio L, Mastriota M, Pieretti S, Celia C, Paolino D, Iannone M, Fresta M, Carafa M

International Journal of Nanomedicine 2014, 9:635-651

Published Date: 24 January 2014

Evaluation of psoralen ethosomes for topical delivery in rats by using in vivo microdialysis

Zhang YT, Shen LN, Zhao JH, Feng NP

International Journal of Nanomedicine 2014, 9:669-678

Published Date: 23 January 2014

Biocompatibility of magnetic Fe3O4 nanoparticles and their cytotoxic effect on MCF-7 cells

Chen DZ, Tang QS, Li XD, Zhou XJ, Zang J, Xue WQ, Xiang JY, Guo CQ

International Journal of Nanomedicine 2012, 7:4973-4982

Published Date: 14 September 2012

Association between calcifying nanoparticles and placental calcification

Guo Y, Zhang D, Lu H, Luo S, Shen X

International Journal of Nanomedicine 2012, 7:1679-1686

Published Date: 27 March 2012

Organic functionalization of single-walled carbon nanotubes (SWCNTs) with some chemotherapeutic agents as a potential method for drug delivery

Ardeshir Khazaei, Mohammad Navid Soltani Rad, Maryam Kiani Borazjani

International Journal of Nanomedicine 2010, 5:639-645

Published Date: 30 August 2010

Gold nanoparticle–choline complexes can block nicotinic acetylcholine receptors

Chur Chin, In Kyeom Kim, Dong Yoon Lim, et al

International Journal of Nanomedicine 2010, 5:315-321

Published Date: 19 April 2010