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Analysis of the metabolic properties of maintenance hemodialysis patients with glucose-added dialysis based on high performance liquid chromatography quadrupole time-of-flight mass spectrometry

Authors Cui L, Meng Y, Xu D, Feng YY, Chen GY, Hu B, Feng GJ, Yin LH

Received 8 June 2013

Accepted for publication 19 September 2013

Published 29 October 2013 Volume 2013:9 Pages 417—425

DOI https://doi.org/10.2147/TCRM.S49634

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 4

Li Cui,1 Yu Meng,2 Dan Xu,2 Yanyan Feng,2 Gangyi Chen,3 Bo Hu,2 Guijuan Feng,4 Lianghong Yin2

1Xi'xiang People's Hospital Affiliated to Guangdong Medical College, Shenzhen, People's Republic of China; 2Nephrology Department of the First Hospital Affiliated to Ji'nan University, Guangzhou, People's Republic of China; 3First Hospital of Guangzhou University of Chinese Medicine, 4Assisted Reproductive Centre of the First Hospital Affiliated to Ji'nan University, Guangzhou, People's Republic of China

Abstract: The purpose of this study was to compare the metabolic properties of maintenance hemodialysis patients treated with glucose-containing and glucose-free dialysate using metabonomics. Pre- and post-dialysis serum samples from group G (−) using glucose-free dialysate, and group G (+) using glucose-added dialysate (glucose levels were 5.5 mmol/L) were analyzed and tested with high performance liquid chromatography quadrupole time-of-flight mass spectrometry. Orthogonal signal correction–partial least squares discriminate analysis revealed a significant difference in the post-dialysis metabolic properties between samples from the G (−) and G (+) groups, and concentrations of leucine and dihydroxyprostaglandin F2α were higher in the G (+) group than in the G (−) group. However, markers of reactive lipid mobilization and amino acid release, such as bile acids, aspartate, and valine, were lower in the G (+) group than in the G (−) group. There were no significant differences in excitatory neurotransmitters aspartate and phosphorylated anandamide. Use of liquid chromatography-tandem mass spectrometry metabonomics indicated that using glucose-added dialysate was superior to glucose-free dialysate in the protection of the central nervous system of maintenance hemodialysis patients, but had potential risks in stimulating oxidative stress.

Keywords: hemodialysis, metabonomics, glucose-added dialysate

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