Back to Journals » International Journal of Nanomedicine » Volume 10 » Issue 1

Amyloids in solid-state nuclear magnetic resonance: potential causes of the usually low resolution

Authors Espargaró A, Busquets MA, Estelrich J, Sabate R

Received 27 May 2015

Accepted for publication 18 July 2015

Published 9 November 2015 Volume 2015:10(1) Pages 6975—6983


Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Govarthanan Muthusamy

Peer reviewer comments 3

Editor who approved publication: Dr Thomas J Webster

Alba Espargaró, Maria Antònia Busquets, Joan Estelrich, Raimon Sabate

Department of Physical Chemistry, School of Pharmacy, Institute of Nanoscience and Nanotechnology (IN2UB), University of Barcelona, Barcelona, Spain

Abstract: Amyloids are non-crystalline and insoluble, which imply that the classical structural biology tools, ie, X-ray crystallography and solution nuclear magnetic resonance (NMR), are not suitable for their analysis. In the last years, solid-state NMR (ssNMR) has emerged as an alternative tool to decrypt the structural signatures of amyloid fibrils, providing major contributions to our understanding of molecular structures of amyloids such as β-amyloid peptide associated with Alzheimer’s disease or fungal prions, among others. Despite this, the wide majority of amyloid fibrils display low resolution by ssNMR. Usually, this low resolution has been attributed to a high disorder or polymorphism of the fibrils, suggesting the existence of diverse elementary β-sheet structures. Here, we propose that a single β-sheet structure could be responsible for the broadening of the line widths in the ssNMR spectra. Although the fibrils and fibers consist of a single elementary structure, the angle of twist of each individual fibril in the mature fiber depends on the number of individual fibrils as well as the fibril arrangement in the final mature fiber. Thus, a wide range of angles of twist could be observed in the same amyloid sample. These twist variations involve changes in amino acid alignments that could be enough to limit the ssNMR resolution.

Keywords: amyloid, fibril, misfolding, β-structure, ssNMR, NMR, β-sheet

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]


Readers of this article also read:

Assessing the axonal translocation of CeO2 and SiO2 nanoparticles in the sciatic nerve fibers of the frog: an ex vivo electrophysiological study

Kastrinaki G, Samsouris C, Kosmidis EK, Papaioannou E, Konstandopoulos AG, Theophilidis G

International Journal of Nanomedicine 2015, 10:7089-7096

Published Date: 19 November 2015

Intracellular targeted co-delivery of shMDR1 and gefitinib with chitosan nanoparticles for overcoming multidrug resistance

Yu XW, Yang G, Shi YJ, Su C, Liu M, Feng B, Zhao L

International Journal of Nanomedicine 2015, 10:7045-7056

Published Date: 12 November 2015

Design and evaluation of an intravesical delivery system for superficial bladder cancer: preparation of gemcitabine HCl-loaded chitosan–thioglycolic acid nanoparticles and comparison of chitosan/poloxamer gels as carriers

Ay Şenyiğit Z, Karavana SY, İlem-Özdemir D, Çalışkan Ç, Waldner C, Şen S, Bernkop-Schnürch A, Baloğlu E

International Journal of Nanomedicine 2015, 10:6493-6507

Published Date: 14 October 2015

Anticancer activities of self-assembled molecular bowls containing a phenanthrene-based donor and Ru(II) acceptors

Kim I, Song YH, Singh N, Jeong YJ, Kwon JE, Kim H, Cho YM, Kang SC, Chi KW

International Journal of Nanomedicine 2015, 10:143-153

Published Date: 25 August 2015

Migraine: treatments, comorbidities, and quality of life, in the USA

Malone CD, Bhowmick A, Wachholtz AB

Journal of Pain Research 2015, 8:537-547

Published Date: 12 August 2015

Magnetic removal of Entamoeba cysts from water using chitosan oligosaccharide-coated iron oxide nanoparticles

Shukla S, Arora V, Jadaun A, Kumar J, Singh N, Jain VK

International Journal of Nanomedicine 2015, 10:4901-4917

Published Date: 31 July 2015