Back to Journals » International Journal of Nanomedicine » Volume 6

AFM, ESEM, TEM, and CLSM in liposomal characterization: a comparative study

Authors Barbara Ruozi, Daniela Belletti, Andrea Tombesi, et al

Published 14 March 2011 Volume 2011:6 Pages 557—563

DOI https://doi.org/10.2147/IJN.S14615

Review by Single-blind

Peer reviewer comments 3

Barbara Ruozi1, Daniela Belletti1, Andrea Tombesi2, Giovanni Tosi1, Lucia Bondioli1, Flavio Forni1, Maria Angela Vandelli1
1Department of Pharmaceutical Sciences, University of Modena and Reggio Emilia, Modena, Italy; 2C.I.G.S., University of Modena and Reggio Emilia, Modena, Italy

Abstract: An outstanding aspect of pharmaceutical nanotechnology lies in the characterization of nanocarriers for targeting of drugs and other bioactive agents. The development of microscopic techniques has made the study of the surface and systems architecture more attractive. In the field of pharmaceutical nanosystems, researchers have collected vital information on size, stability, and bilayer organization through the microscopic characterization of liposomes. This paper aims to compare the results obtained by atomic force microscopy, environmental scanning electron microscopy, transmission electron microscopy, and confocal laser scanning microscopy to point out the limits and advantages of these applications in the evaluation of vesicular systems. Besides this comparative aim, our work proposes a simple confocal laser scanning microscopy procedure to rapidly and easily detect the liposomal membrane.
Keywords: atomic force microscopy, transmission electron microscopy, environmental scanning electron microscopy, confocal laser scanning microscopy

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]

 

Readers of this article also read:

Synthesis of silver nanoparticles by using tea leaf extract from Camellia Sinensis

Loo YY, Chieng BW, Nishibuchi M, Radu S

International Journal of Nanomedicine 2012, 7:4263-4267

Published Date: 2 August 2012

Enhanced absorption of hydroxysafflor yellow A using a self-double-emulsifying drug delivery system: in vitro and in vivo studies

Lv LZ, Tong CQ, Lv Q, Tang XJ, Li LM, Fang QX, Yu J, Han M, Gao JQ

International Journal of Nanomedicine 2012, 7:4099-4107

Published Date: 30 July 2012

Long-circulating delivery of bioactive polysaccharide from radix ophiopogonis by PEGylation

Lin X, Wang ZJ, Huang F, Liang S, Shen L, Feng Y, Ruan KF

International Journal of Nanomedicine 2011, 6:2865-2872

Published Date: 11 November 2011

Physicochemical properties and biocompatibility of a polymer-paclitaxel conjugate for cancer treatment

Yang D, Van S, Liu J, Wang J, Jiang X, Wang Y, Yu L

International Journal of Nanomedicine 2011, 6:2557-2566

Published Date: 21 October 2011

Biomimetic component coating on 3D scaffolds using high bioactivity of mesoporous bioactive ceramics

Yun HS, Kim SH, Khang DW, Choi JI, Kim HH, Kang MJ

International Journal of Nanomedicine 2011, 6:2521-2531

Published Date: 21 October 2011

Chemical nature and structure of organic coating of quantum dots is crucial for their application in imaging diagnostics

Bakalova R, Zhelev Z, Kokuryo D, Spasov L, Aoki I, Saga T

International Journal of Nanomedicine 2011, 6:1719-1732

Published Date: 18 August 2011

Effect of size and processing method on the cytotoxicity of realgar nanoparticles in cancer cell lines

Zhao W, Lu X, Yuan Y, Liu C, Yang B, Hong H, Wang G, Zeng F

International Journal of Nanomedicine 2011, 6:1569-1577

Published Date: 2 August 2011