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Adhesion, proliferation, and apoptosis in different molecular portraits of breast cancer treated with silver nanoparticles and its pathway-network analysis

Authors Rodríguez-Razón CM, Yañez-Sánchez I, Ramos-Santillan VO, Velásquez-Ordóñez C, Gutiérrez-Rubio SA, García-García MR, López-Roa RI, Sánchez-Hernández PE, Daneri-Navarro A, García-Iglesias T

Received 21 September 2017

Accepted for publication 28 November 2017

Published 22 February 2018 Volume 2018:13 Pages 1081—1095

DOI https://doi.org/10.2147/IJN.S152237

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Alexander Kharlamov

Peer reviewer comments 3

Editor who approved publication: Dr Thomas Webster


Christian M Rodríguez-Razón,1 Irinea Yañez-Sánchez,2 Vicente O Ramos-Santillan,1 Celso Velásquez-Ordóñez,2 Susan A Gutiérrez-Rubio,1 Maritza R García-García,3 Roció I López-Roa,1 Pedro E Sánchez-Hernández,1 Adrian Daneri-Navarro,1 Trinidad García-Iglesias1

1Laboratory of Immunology and Institute of Experimental and Clinical Therapeutics, Department of Physiology, University Center of Health Sciences, University of Guadalajara, Jalisco, Mexico; 2Center for Research in Nanosciences and Nanotechnology, Department of Natural and Exact Sciences, University Center of the Valleys, University of Guadalajara, Jalisco, Mexico; 3Department of Health Sciences, University Center of the High University of Guadalajara, Jalisco, Mexico

Background: Silver nanoparticles (AgNPs) have attracted considerable attention due to the variety of their applications in medicine and other sciences. AgNPs have been used in vitro for treatment of various diseases, such as hepatitis B and herpes simplex infections as well as colon, cervical, and lung cancers. In this study, we assessed the effect on proliferation, adhesion, and apoptosis in breast cancer cell lines of different molecular profiles (MCF7, HCC1954, and HCC70) exposed to AgNPs (2–9 nm).
Methods: Breast cancer cell lines were incubated in vitro; MTT assay was used to assess proliferation. Adhesion was determined by real-time analysis with the xCELLingence system. Propidium iodide and fluorescein isothiocyanate-Annexin V assay were used to measure apoptosis. The transcriptome was assessed by gene expression microarray and Probabilistic Graphical Model (PGM) analyses.
Results: The results showed a decreased adhesion in breast cancer cell lines and the control exposed to AgNPs was noted in 24 hours (p≤0.05). We observed a significant reduction in the proliferation of MCF7 and HCC70, but not in HCC1954. Apoptotic activity was seen in all cell lines exposed to AgNPs, with an apoptosis percentage of more than 60% in cancer cell lines and less than 60% in the control. PGM analysis confirmed, to some extent, the effects of AgNPs primarily on adhesion by changes in the extracellular matrix.
Conclusion: Exposure to AgNPs causes an antiproliferative, apoptotic, and anti-adhesive effect in breast cancer cell lines cultured in vitro. More research is needed to evaluate the potential use of AgNPs to treat different molecular profiles of breast cancer in humans.

Keywords: silver nanoparticles, breast cancer, adhesion, apoptosis, transcriptome

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