A TEM-traceable physiologically functional gold nanoprobe that permeates non-endocytic cells
Authors Berberian MV, Pocognoni CA, Mayorga LS
Received 14 March 2018
Accepted for publication 3 July 2018
Published 28 November 2018 Volume 2018:13 Pages 8075—8086
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Andrew Yee
Peer reviewer comments 3
Editor who approved publication: Dr Thomas Webster
Maria Victoria Berberian,1 Cristian A Pocognoni,2 Luis S Mayorga1,2
1Institute of Histology and Embryology of Mendoza – CONICET, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Cuyo, Mendoza, Argentina; 2Institute of Histology and Embryology of Mendoza – CONICET, Facultad de Ciencias Médicas, Universidad Nacional de Cuyo, Mendoza, Argentina
Background: Nanoparticles’ intracellular fate requires proper internalization. Most cells make use of a battery of internalization pathways, but some are practically sealed, as they lack the biochemical machinery for cellular intake. Non-endocytic cells, such as mammals’ spermatozoa, challenge standard drug-delivery strategies.
Purpose: In this article, we present a gold nanoprobe that permeates the external and internal membranes of human sperm.
Methods: Our design makes use of a gold nanoparticle functionalized with a membrane-permeable cysteine-rich recombinant protein. The chimeric protein contains two units of physiologically active metallothioneins (MT) that also provide binding motifs to gold and a cell-penetrating-peptide sequence (CPP) that confers cell permeability to the nanoparticle.
Results: Transmission electron microscopy, indirect immunofluorescence, and functional assays show that the nanoprobe is readily internalized in sperm, without compromising cell integrity, while preserving MT’s physiological activity. Our findings highlight the potential of CPP-functionalized nanogold for investigating the physiology of otherwise impermeable non-endocytic cells.
Keywords: human sperm, metallothionein, gold nanoparticles functionalization, cell-penetrating peptides, transmission electron microscopy
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