Back to Journals » International Journal of Nanomedicine » Volume 10 » Issue 1

A single dose of dexamethasone encapsulated in polyethylene glycol-coated polylactic acid nanoparticles attenuates cisplatin-induced hearing loss following round window membrane administration

Authors Sun CL, Wang XL, Zheng ZZ, Chen DY, Wang XQ, Shi F, Yu DH, Wu H

Received 21 November 2014

Accepted for publication 9 March 2015

Published 14 May 2015 Volume 2015:10(1) Pages 3567—3579

DOI https://doi.org/10.2147/IJN.S77912

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 4

Editor who approved publication: Dr Lei Yang

Changling Sun,1,3,* Xueling Wang,1,* Zhaozhu Zheng,2 Dongye Chen,1 Xiaoqin Wang,2 Fuxin Shi,1 Dehong Yu,1 Hao Wu1

1Department of Otolaryngology–Head and Neck Surgery, Xinhua Hospital, Ear Institute, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Translational Medicine on Ear and Nose Diseases, Shanghai, 2National Engineering Laboratory for Modern Silk, Soochow University, Suzhou, 3Department of Otolaryngology–Head and Neck Surgery, Affiliated Hospital of Jiangnan University, The Fourth People’s Hospital of Wuxi City, Wuxi, People’s Republic of China

*These authors have contributed equally to this work

Abstract: This study aimed to investigate the sustained drug release properties and hearing protection effect of polyethylene glycol-coated polylactic acid (PEG-PLA) stealth nanoparticles loaded with dexamethasone (DEX). DEX was fabricated into PEG-PLA nanoparticles using an emulsion and evaporation technique, as previously reported. The DEX-loaded PEG-PLA nanoparticles (DEX-NPs) had a hydrodynamic diameter of 130±4.78 nm, and a zeta potential of -26.13±3.28 mV. The in vitro release of DEX from DEX-NPs lasted 24 days in phosphate buffered saline (pH 7.4), 5 days in artificial perilymph (pH 7.4), and 1 day in rat plasma. Coumarin 6-labeled NPs placed onto the round window membrane (RWM) of guinea pigs penetrated RWM quickly and accumulated to the organs of Corti, stria vascularis, and spiral ganglion cells after 1 hour of administration. The DEX-NPs locally applied onto the RWM of guinea pigs by a single-dose administration continuously released DEX in 48 hours, which was significantly longer than the free DEX that was cleared out within 12 hours after administration at the same dose. Further functional studies showed that locally administrated single-dose DEX-NPs effectively preserved outer hair cells in guinea pigs after cisplatin insult and thus significantly attenuated hearing loss at 4 kHz and 8 kHz frequencies when compared to the control of free DEX formulation. Histological analyses indicated that the administration of DEX-NPs did not induce local inflammatory responses. Therefore, prolonged delivery of DEX by PEG-PLA nanoparticles through local RWM diffusion (administration) significantly protected the hair cells and auditory function in guinea pigs from cisplatin toxicity, as determined at both histological and functional levels, suggesting the potential therapeutic benefits in clinical applications.

Keywords: stealth nanoparticles, dexamethasone, single-dose administration, cisplatin-induced hearing loss

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]

 

Readers of this article also read:

Nanostructured lipid system as a strategy to improve the anti-Candida albicans activity of Astronium sp.

Bonifácio BV, Ramos MAS, Silva PB, Negri KMS, Lopes EO, Souza LP, Vilegas W, Pavan FR, Chorilli M, Bauab TM

International Journal of Nanomedicine 2015, 10:5081-5092

Published Date: 10 August 2015

Central nervous system toxicity of metallic nanoparticles

Feng XL, Chen AJ, Zhang YL, Wang JF, Shao LQ, Wei LM

International Journal of Nanomedicine 2015, 10:4321-4340

Published Date: 3 July 2015

Targeted tumor imaging of anti-CD20-polymeric nanoparticles developed for the diagnosis of B-cell malignancies

Capolla S, Garrovo C, Zorzet S, Lorenzon A, Rampazzo E, Spretz R, Pozzato G, Núñez L, Tripodo C, Macor P, Biffi S

International Journal of Nanomedicine 2015, 10:4099-4109

Published Date: 22 June 2015

Dual-color immunofluorescent labeling with quantum dots of the diabetes-associated proteins aldose reductase and Toll-like receptor 4 in the kidneys of diabetic rats

Liu XM, Hu R, Lian HW, Liu Y, Liu J, Liu JW, Lin GM, Liu LW, Duan XJ, Yong KT, Ye L

International Journal of Nanomedicine 2015, 10:3651-3662

Published Date: 20 May 2015

Amalgamation of complex iron(III) ions and iron nanoclusters with MWCNTs as a route to potential T2 MRI contrast agents

Kuźnik N, Tomczyk MM, Wyskocka M, Przypis L, Herman AP, Jędrysiak R, Koziol KK, Boncel S

International Journal of Nanomedicine 2015, 10:3581-3591

Published Date: 14 May 2015

Reduction-responsive cross-linked stearyl peptide for effective delivery of plasmid DNA

Yao C, Tai Z, Wang X, Liu J, Zhu Q, Wu X, Zhang L, Zhang W, Tian J, Gao Y, Gao S

International Journal of Nanomedicine 2015, 10:3403-3416

Published Date: 8 May 2015

Chloramphenicol encapsulated in poly-ε-caprolactone–pluronic composite: nanoparticles for treatment of MRSA-infected burn wounds

Kalita S, Devi B, Kandimalla R, Sharma KK, Sharma A, Kalita K, Kataki AC, Kotoky J

International Journal of Nanomedicine 2015, 10:2971-2984

Published Date: 15 April 2015

Enhanced local bioavailability of single or compound drugs delivery to the inner ear through application of PLGA nanoparticles via round window administration

Cai H, Wen X, Wen L, Tirelli N, Zhang X, Zhang Y, Su H, Yang F, Chen G

International Journal of Nanomedicine 2014, 9:5591-5601

Published Date: 1 December 2014