Back to Journals » International Journal of Nanomedicine » Volume 7

A sandwich-type DNA electrochemical biosensor for hairpin-stem-loop structure based on multistep temperature-controlling method

Authors Hong GL, Liu YH, Chen W, Weng SH, Liu QC, Liu AL, Zheng DX, Lin XH

Received 20 June 2012

Accepted for publication 25 July 2012

Published 14 September 2012 Volume 2012:7 Pages 4953—4960

DOI https://doi.org/10.2147/IJN.S35177

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 3

Guolin Hong,1,2,* Yinhuan Liu,1,* Wei Chen,3,5 Shaohuang Weng,3,5 Qicai Liu,4 Ailin Liu,3,5 Daoxin Zheng,1 Xinhua Lin,3,5

1
Department of Laboratory Medicine, The Fuzhou Second Affiliated Hospital of Xiamen University, Fuzhou, China; 2Department of Laboratory Medicine, Medical Technology and Engineering College of Fujian Medical University, Fuzhou, China; 3Department of Pharmaceutical Analysis, Pharmacy College of Fujian Medical University, Fuzhou, China; 4Department of Laboratory Medicine, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China; 5Nano Medical Technology Research Institute, Fujian Medical University, Fuzhou, China

*Guolin Hong and Yinhuan Liu contributed equally to the present study

Abstract: A highly sensitive and selective method for amplified electrochemical detection for hairpin-stem-loop structured target sequences was developed based on the temperature regulation of DNA hybrids on a sandwich-type electrochemical DNA sensor. Multistep hybridization was applied to promote the hybridization efficiency of each section of sandwich structure. The results showed that both multistep and temperature-controlling hybridization techniques were both especially made to fabricate the sensor for the tendency of internal hybridization of target gene sequences. This strategy provides significantly enhanced hybridization efficiency and sequence specificity of electrochemical detection.

Keywords: sandwich-type biosensor, structured target, multistep temperature-controlling, DNA hybridization, tubercle bacillus

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]

 

Readers of this article also read:

Antimicrobial activity of metal oxide nanoparticles against Gram-positive and Gram-negative bacteria: a comparative study

Azam A, Ahmed AS, Oves M, Khan MS, Habib SS, Memic A

International Journal of Nanomedicine 2012, 7:6003-6009

Published Date: 5 December 2012

A new approach to the treatment of recurrent aphthous stomatitis with bioadhesive gels containing cyclosporine A solid lipid nanoparticles: in vivo/in vitro examinations

Karavana SY, Gökçe EH, Rençber S, Özbal S, Pekçetin Ç, Güneri P, Ertan G

International Journal of Nanomedicine 2012, 7:5693-5704

Published Date: 9 November 2012

Long-term MRI tracking of dual-labeled adipose-derived stem cells homing into mouse carotid artery injury

Qin JB, Li KA, Li XX, Xie QS, Lin JY, Ye KC, Jiang ME, Zhang GX, Lu XW

International Journal of Nanomedicine 2012, 7:5191-5203

Published Date: 2 October 2012

Mannan-modified Ad5-PTEN treatment combined with docetaxel improves the therapeutic effect in H22 tumor-bearing mice

Liu Z, Li J, Li J, Huang J, Ke F, Qi Q, Jiang X, Zhong Z

International Journal of Nanomedicine 2012, 7:5039-5049

Published Date: 17 September 2012

Enhanced cellular uptake and long-term retention of chitosan-modified iron-oxide nanoparticles for MRI-based cell tracking

Bakhru SH, Altiok E, Highley C, Delubac D, Suhan J, Hitchens TK, Ho C, Zappe S

International Journal of Nanomedicine 2012, 7:4613-4623

Published Date: 22 August 2012

Evaluation of the genotoxicity of cellulose nanofibers

de Lima R, Feitosa LO, Maruyama CR, Barga MA, Yamawaki PC, Vieira IJ, Teixeira EM, Corrêa AC, Mattoso LH, Fraceto LF

International Journal of Nanomedicine 2012, 7:3555-3565

Published Date: 11 July 2012

A novel lipid-based nanomicelle of docetaxel: evaluation of antitumor activity and biodistribution

Ma M, Hao Y, Liu N, Yin Z, Wang L, Liang X, Zhang X

International Journal of Nanomedicine 2012, 7:3389-3398

Published Date: 4 July 2012

Supramolecular micellar nanoaggregates based on a novel chitosan/vitamin E succinate copolymer for paclitaxel selective delivery

Lian H, Sun J, Yu YP, Liu YH, Cao W, Wang YJ, Sun YH, Wang SL, He ZG

International Journal of Nanomedicine 2011, 6:3323-3334

Published Date: 12 December 2011

Practical preparation procedures for docetaxel-loaded nanoparticles using polylactic acid-co-glycolic acid

Keum CG, Noh YW, Baek JS, Lim JH, Hwang CJ, Na YG, Shin SC, Cho CW

International Journal of Nanomedicine 2011, 6:2225-2234

Published Date: 7 October 2011