Back to Browse Journals » Drug Design, Development and Therapy » Volume 2

A safety-modified SV40 Tag developed for human cancer immunotherapy

Authors Stephanie S Tseng-Rogenski, Mohamed S Arredouani, June F Escara-Wilke, Yilin C Neeley, Michael J Imperiale et al

Published 8 January 2008 Volume 2008:2 Pages 17—24

Stephanie S Tseng-Rogenski1, Mohamed S Arredouani2, June F Escara-Wilke1, Yilin C Neeley1, Michael J Imperiale3, Martin G Sanda1,2

1Department of Urology, School of Medicine, University of Michigan, Ann Arbor, MI, USA; 2Department of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA; 3Department of Microbiology and Immunology, School of Medicine, University of Michigan, Ann Arbor, MI, USA

Abstract: Simian virus 40 (SV40)-like DNA sequences have been found in a variety of human tumors, raising the possibility that strategies targeting SV40 may provide a potential avenue for immunotherapy directed against SV40 large T Antigen (Tag)-expressing tumors. We generated a recombinant vaccinia (vac-mTag) expressing mTag and herein assessed the ability of mTag to transform cells and to interact with anti-oncoproteins, as well as screened for the presence of potential HLA-A2.1-restricted epitopes within mTag. We found that transfection of cells with mTag did not lead to their transformation. Also, we demonstrated that mTag protein is degraded rapidly in cells. In addition, our work revealed that mTag did not physically interact with certain anti-oncoproteins. Finally, two potential HLA-A2.1-restricted functional epitopes within mTag sequence were identified. Our results show that mTag lacks the oncogenecity of full-length Tag and harbors potential HLA-A2.1-restricted immunogenic epitopes, hence suggesting the safety of vac-mTag for use in cancer immunotherapy.
Keywords: modified SV-40 T antigen, recombinant vaccinia, cancer immunotherapy

Download Article [PDF] 

Readers of this article also read:

The role of sideport incision in astigmatism change after cataract surgery

Theodoulidou S, Asproudis I, Kalogeropoulos C, Athanasiadis A, Aspiotis M

Clinical Ophthalmology 2015, 9:1421-1428

Published Date: 5 August 2015

Prevalence of chronic obstructive pulmonary disease among patients with systemic arterial hypertension without respiratory symptoms

Rabahi MF, Pereira SA, Silva Júnior JL, Rezende AP, Castro da Costa A, Corrêa KS, Conde MB

International Journal of Chronic Obstructive Pulmonary Disease 2015, 10:1525-1529

Published Date: 31 July 2015

Quetiapine for acute bipolar depression: a systematic review and meta-analysis

Suttajit S, Srisurapanont M, Maneeton N, Maneeton B

Drug Design, Development and Therapy 2014, 8:827-838

Published Date: 25 June 2014

Local sustained delivery of acetylsalicylic acid via hybrid stent with biodegradable nanofibers reduces adhesion of blood cells and promotes reendothelialization of the denuded artery

Lee CH, Lin YH, Chang SH, Tai CD, Liu SJ, Chu Y, Wang CJ, Hsu MY, Chang H, Chang GJ, Hung KC, Hsieh MJ, Lin FC, Hsieh IC, Wen MS, Huang Y

International Journal of Nanomedicine 2014, 9:311-326

Published Date: 6 January 2014


Wu Q, Chu M

International Journal of Nanomedicine 2012, 7:4531-4532

Published Date: 15 August 2012

Nimodipine-loaded mixed micelles: formulation, compatibility, pharmacokinetics, and vascular irritability study

Song X, Jiang Y, Ren CJ, Sun X, Zhang Q, Gong T, Zhang ZR

International Journal of Nanomedicine 2012, 7:3689-3699

Published Date: 13 July 2012

Visualization of gold and platinum nanoparticles interacting with Salmonella Enteritidis and Listeria monocytogenes

Ewa Sawosz, André Chwalibog, Jacek Szeliga, et al.

International Journal of Nanomedicine 2010, 5:631-637

Published Date: 30 August 2010

Structure-based design of anticancer prodrug PABA/NO

Xinhua Ji, Ajai Pal, Ravi Kalathur, Xun Hu, Yijun Gu, et al

Drug Design, Development and Therapy 2008, 2:123-130

Published Date: 11 August 2008