A Preliminary Study on the Establishment of the PDTX Model
Authors Liu Y, Zhu YP, Cai MZ, Ke B, Li B, Liu N, Xue Q, Zhan HJ, Deng JY, Zhang L, Hao YP, Wang ZQ, Wang L, Liang H
Received 11 September 2019
Accepted for publication 22 December 2019
Published 17 March 2020 Volume 2020:12 Pages 1969—1979
Checked for plagiarism Yes
Review by Single-blind
Peer reviewer comments 3
Editor who approved publication: Dr Eileen O'Reilly
Yong Liu,1 Yan-Ping Zhu,2 Ming-Zhi Cai,1 Bin Ke,1 Bin Li,1 Ning Liu,1 Qiang Xue,1 Hong-Jie Zhan,1 Jing-Yu Deng,1 Li Zhang,1 Yan-Peng Hao,2 Zhi-Qiang Wang,2 Li Wang,2 Han Liang1
1Department of Gastroenterology, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Centre for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin, Tianjin’s Clinical Research Center for Cancer, Tianjin 300060, People’s Republic of China; 2Nanjing Personal Oncology Biological Technology Co. Ltd., Nanjing 211100, Jiangsu, People’s Republic of China
Correspondence: Han Liang
Department of Gastroenterology, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Centre for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin, Tianjin’s Clinical Research Center for Cancer, Huan-Hu-Xi Road, Ti-Yuan-Bei, He Xi District, Tianjin 300060, People’s Republic of China
Tel +86 22 2334 0123-1061
Fax +86 22 2334 0123
Objective: The current study aims to explore the establishment of the patient-derived tumor xenograft (PDTX) model.
Materials and Methods: Twenty patients with gastric cancer, 10 males and 10 females, were enrolled in the current study. Firstly, the volume, invasion and metastasis of the xenografts were observed. Subsequently, the correlation between tumor tissues of the PDTX mouse model and the patients’ primary tumor tissues was evaluated by pathological H&E staining and immunohistochemistry.
Results: The results showed that the PDTX models corresponding to 15 of the 20 patients were successfully established, and the success rate of PDTX model establishment was 75%. Furthermore, the PDTX models maintained the differentiation degree, morphological characteristics and structural characteristics of tumor cells.
Conclusion: A PDTX model can be used as a substitute for cancer patients in clinical practice and may be suitable for clinical pharmacodynamic screening and new drug development.
Keywords: gastric tumor, PDTX, xenotransplantation model
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