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A molecular toolbox for genetic manipulation of zebrafish

Authors Sassen WA, Köster R

Received 22 October 2014

Accepted for publication 1 January 2015

Published 27 March 2015 Volume 2015:5 Pages 151—163

DOI https://doi.org/10.2147/AGG.S57585

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 7

Editor who approved publication: Dr John Martignetti


Wiebke A Sassen, Reinhard W Köster

Division of Cellular and Molecular Neurobiology, Zoological Institute, Technical University Braunschweig, Braunschweig, Germany

Abstract: Zebrafish is an emerging model system in biomedical research. The high degree of evolutionary conservation in genetics as well as in development allows deciphering the molecular mechanisms underlying vertebrate development and human diseases. Zebrafish offers important advantages as a genetic model organism such as a completely sequenced genome, fast external embryonic development accompanied by nearly perfect transparency, a large number of offspring year round, and molecular accessibility for genetic manipulation. This review discusses established and newly developed approaches for genetic manipulation of zebrafish and their usefulness to understand gene function in various human disorders like heart defects or Alzheimer's disease.

Keywords: zebrafish, genetic manipulation, disease models, transgenes, forward genetics, reverse genetic

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