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A modification of intraluminal middle cerebral artery occlusion/reperfusion model for ischemic stroke with laser Doppler flowmetry guidance in mice

Authors Cai Q, Xu G, Liu J, Wang L, Deng G, Liu J, Chen Z

Received 30 July 2016

Accepted for publication 7 September 2016

Published 3 November 2016 Volume 2016:12 Pages 2851—2858

DOI https://doi.org/10.2147/NDT.S118531

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Colin Mak

Peer reviewer comments 4

Editor who approved publication: Professor Wai Kwong Tang


Qiang Cai,1,* Gang Xu,2,* Junhui Liu,1 Long Wang,1 Gang Deng,1 Jun Liu,3 Zhibiao Chen1

1Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, 2Department of Neurosurgery, Xiantao First People’s Hospital, Xiantao, 3Department of Emergency, The Central Hospital of Wuhan, Wuhan, Hubei, People’s Republic of China

*These authors contributed equally to this work

Abstract: Stroke is one of the common causes of death and disability in the world. The intraluminal middle cerebral artery occlusion/reperfusion (MCAO/R) model is a “gold standard” in surgical ischemic stroke models. Here, we optimized the procedure of this model by ligating on external carotid artery (ECA) stump and two ligatures prepared on internal carotid artery, which could improve the success and survival rate in mice. The results show that ECA approach was superior to common carotid artery approach. Meanwhile, we found that the exposure of pterygopalatine artery was not an essential step for MCAO/R model in mice.

Keywords: middle cerebral artery occlusion, ischemia stroke, animal model, mice

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