A 25-year trace of methicillin-resistant Staphylococcus aureus dissemination in a geriatric hospital in Japan
Fuminori Sakai1, Hideaki Hanaki2, Kazunari Barada4, Yuriko Hirao1, Takashi Inamatsu5, Taiji Nakae2, Keisuke Sunakawa3
1Laboratory of Infectious Diseases, Graduate School of Infection Control Sciences, 2Research Center for Anti-Infectious Drugs, Kitasato Institute, 3Department of Research Project Studies, Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan; 4Department of Pharmacy, General Ota Hospital, Society of Health Insurance of Fuji Heavy Industries Ltd, Gunma, Japan; 5Department of Infectious Diseases, Tokyo Metropolitan Geriatric Hospital, Tokyo, Japan
Abstract: We analyzed 218 strains of methicillin-resistant Staphylococcus aureus (MRSA) isolated from the septicemia patients in a geriatric hospital for 25 years. These strains were classified into 11 major DNA types, A through K, and 27 minor types. The strains belonging to group A and B isolated before 1990 were susceptible to imipenem (IPM), fluoroquinolone, and most other antibiotics tested, except that they were markedly resistant to gentamicin. Strains mostly isolated in 1985 and thereafter were classified into group C through K, and they were mainly resistant to IPM, fluoroquinolones, and clindamycin. Analysis of the MRSA marker gene, staphylococcal cassette chromosome mec (SCCmec), of these strains revealed that the strains in groups A and B had mainly type IV and type I, respectively, and that strains in groups C through J had mainly type II. These results suggested that the strains holding type II SCCmec were resistant to IPM, fluoroquinolone, and clindamycin and they were dominant-resistant type after late 1980s. The antibiotic resistance profiles of MRSA dramatically changed during late 1980s, and these were correlated with the SCCmec types. The lesson from this study would be that consistent execution of surveillance study is needed to update the resistant profiles.
Keywords: methicillin-resistant Staphylococcus aureus, DNA typing, antibiotic resistance, survey, geriatric hospital
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